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1994 Fiscal Year Final Research Report Summary

Preparation of ^<99m>Tc radiopharmaceuticals using macroreticular Sn (II) complex

Research Project

Project/Area Number 04557104
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Physical pharmacy
Research InstitutionKumamoto University

Principal Investigator

NAKAYAMA Morio  Kumamoto University, Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (60164373)

Co-Investigator(Kenkyū-buntansha) YOMOTA Isamu  Daiichi Radioisotope Laboratories, Reseracher, 研究開発部, 研究員
TOMIGUCHI Seiji  Kumamoto University, Pharmaceutical Sciences, Lecturerer, 医学部附属病院, 講師 (20172182)
HARADA Kumiko  Kumamoto University, Pharmaceutical Sciences, Assistant Professor, 薬学部, 助手 (70150547)
Project Period (FY) 1992 – 1994
KeywordsTechnetium / Macromolecular Sn (II) complex / Aminophosphonic acid groups / Monoclonal anitibody / Immunoglobulin
Research Abstract

Stannous chloride (SnCl_2) has the disadvantages of being easily hydrolyzed and oxidized, but it is included in most commercial kits as an appropriate reducing agent in the preparation of ^<99m>Tc radiopharmaceuticals. The replacement of SnCl_2 by the insoluble macromolecular Sn (II) (R-Sn) complex was proposed as a way to resolve some of the problems caused by a large excess of SnCl_2 Macroreticular chelating ion exchange resins having an adsorption ability for Sn (II) were investigated for the development of R-Sn complex. Among them, a chelating resin containing aminophosphonic acid groups showed a high capacity for Sn (II) , which bound strongly to the resin by chelation. The use of the R-Sn complex was expected to minimize Sn (II) comtamination of the ^<99m>Tc labeling solution and be effectively used as a reducing agent for ^<99m>Tc labeling of proteins containing sulfhydryl groups. So, the R-Sn complex was applied to the direct ^<99m>Tc labeling of human immunoglobulin (IgG) to minimize the influence of Sn (II). ^<99m>Tc labeling was achieved at greater than 90% yield simply by the short-term mixing of IgGa containing>2 -SH groups per IgG molecule, ^<99m>Tc pertechnetate, and the R-Sn complex in pH 7 solution. The ^<99m>Tc-IgGa obtained by this labeling method has high stability based on the thiol-specific binding of ^<99m>Tc without transchelation from another weakly bound ^<99m>Tc-complex.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 中山 守雄: "^<99m>Tc還元のための高分子スズ錯体の開発と血清蛋白質の^<99m>Tc標識への応用" 放射線生物研究. 29. 349-356 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中山 守雄: "Insoluble Macromolecular Sn(II) complex for the ^<99m>Tc labeling of protein-bearing mercapto groups." J.Nucl.Biol.Med.38. 459-460 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中山 守雄: "Direct ^<99m>Tc Labeling of human imunoglobulin with insoluble macromolecular Sn(II) complex." Nucl.Med.Biol.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morio Nakayama: "Development of macromolecular Sn (II) complex for reduction of ^<99m>Tc and the application to ^<99m>Tc labeling of serum protein." Radiation and Biology Reserach. 29. 349-356 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morio Nakayama: "Insoluble Macromolecular Sn(II)complex for the ^<99m>Tc labeling of protein-bearing mercapto groups." J.Nucl.Biol.Med.38. 459-460 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mrio Nakayama: "Direct ^<99m>Tc Labeling of human imunoglobulin with insoluble macromolecular Sn (II) complex." Nucl.Med.Biol. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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