1993 Fiscal Year Final Research Report Summary
The establishment of the standard method for rat embryo-cryopreservation method and for the network.
| Project/Area Number |
04558012
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
KASAI Noriyuki HOKKAIDO UNIV.ASSOCIATE PROFESSOR, 医学部, 助教授 (60001947)
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| Co-Investigator(Kenkyū-buntansha) |
HIRABAYASHI Masumi YS NEW TECHNOLOGY SENIOR RESEARCHER, 研究員
YAMASHITA Tadashi HOKKAIDO UNIV.FACULT.OF VET.ASSISTANT PROFESSOR, 獣医学部, 助手 (30220338)
KASAI Magosaburou KOCHI UNIV.FACULT.OF AGRI.PROFESSOR, 農学部, 教授 (60152617)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Rat / Obtaining embryos / Embryo cryopreservation / Vitrification / Cryoprotectant / Net work / 2Cell embryo / Slow freezing method |
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| Research Abstract |
Vitrification method for rat embryo cryopresearvation has not been established yet. In addition, it is not clear which facilitles are cryopresearving the rat embryos. The purpose of this research is as follows ;
1. The establishment of the standard method for rat embryo-cryopresear vation.
2. The development of the vitrification method for rat embryos.
3. The establishment of the effective method for obtaining rat embryos.
4. The establishment of the database and the network system for rat embryo-cryopresearvation in Japan.
The best method for rat embryo-cryopresearvation is the method that Mizuno et al. developed. They reported and we also think that the 2 cell embryos are the best stage for cryopresearvation.
Hirabayashi et al. and Kasai et al. improved the vitrification methods previously reported. But the methods have not been completed.
Ishikawa et al. and Yamazaki et al. improved the methods for obtaining rat embryo-cryopresearvation by the induced ovulation with PMSG and hCG and these methods are useful for practice.
The database and the network for rat embryo-cryopresearvation have not been established yet in this study. We will continue to do these purpose.
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[Publications] Miyoshi,I.,Yamashita,T.,Aoyama,S.,Sasaki,N.,Maki,K.,Kasai,N: "Effect of environmental factors on the yield of mouse embryos obtained after hormone induced superovalation" Lab.Anim.Sci.43. 642-645 (1993)
Description
「研究成果報告書概要(和文)」より
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[Publications] Yamazaki,H.,Yamashita,T.,Miyoshi,I.,Osanai,T.,Yoshiki,T.,Kasai,N: "Determination of optimal conditions for obtaining rats embryos by the induction of superovalation" Lab.Anim.Sci.(in submission). (1994)
Description
「研究成果報告書概要(和文)」より
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[Publications] Kasai, M., Hiraisi, M., Nureki, I., Zhu, S.E., Sakurai, T., and Machida, T.: "Influence of the mucin coat on the survival of rabbit and mouse embryos stored at 0。C." J.Exp.zool.267. 344-349 (1993)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Miyosi, I., Yamasita, Y., Aoyama, S., Sasaki, N., Maki, K., and Kasai, N.: "Effect of environmental factors on the yield of mouse embryos obtained after hormone-induced superovulation." Lab.Anim.Sci.43. 642-645 (1993)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yamazaki, H., Yamashita, T., Miyoshi, I., Osanai, T., Yoshiki, T., Kasai, N.: "Determination of optimal conditions for obtaining rats embryos by the induction of superovulation." Lab.Anim.Sci.(In Submission). (1994)
Description
「研究成果報告書概要(欧文)」より
