1993 Fiscal Year Final Research Report Summary
Generation of a new mouse model for transplantation of human cells by utilizing an anti-IL-2 receptor beta chain monoclonal antibody
| Project/Area Number |
04558014
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | Tokyo Metropolitan Institute for Medical Science |
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Principal Investigator |
MIYASAKA Masayuki Tokyo Metropolitan Institute of Medical Science, Dept.Immunol., Head, 免疫研究部門, 研究員 (50064613)
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| Co-Investigator(Kenkyū-buntansha) |
NAWA Yukifumi Miyazaki University Medical School Dept.Parasitol., Professor, 寄生虫学教室, 教授 (10040172)
UEYAMA Yoshihito Tokai University Medical School Dept.Pathol., Associate Professor, 病理学教室, 助教授 (30072408)
URANO Kouji Institute for Experimental Animals Research Scientist, 研究員 (80213510)
KITAMURA Fujiko TMIMS, Dept.Immunol., Research Member, 免疫研究部門, 研究員 (90124453)
TANAKA Toshiyuki TMIMS, Dept.Immunol., Research Member, 免疫研究部門, 研究員 (30217054)
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| Project Period (FY) |
1992 – 1993
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| Keywords | IL-2 receptor beta chain / NK cell / SCID mouse / SCID-Hu |
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| Research Abstract |
By takeing advantage of the fact that natural killer (NK) cells can be eliminated specifically for long-term in vivo by a single i.p. injection of monoclonal antibody against IL-2 receptor beta chain (anti-IL-2Rbeta), we attempted to generate a mouss model in which various types of human cells can be readily transplanted. We found that injection of the anti-IL-2Rbeta into SCID mice induces long-term elimination of NK cells, thus generating T-, B- and NK-deficient mice. ATL cells that do not normally grow in untreated SCID mice did grow well in such antibody-treated NK-deficient SCID mice, raising an interesting possibility that this in vivo model can be used for screening and testing the efficacy of anti-ATL drug candidates. This antibody may be also be beneficial for generation of SCID-Hu.
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[Publications] Tanaka,T.,Kitamura,F.,Nagasaka,Y.,Kuida,K.,Suwa,H.and Miyasaka,M.: "Selective long-term elimination of natural killer cells in vivo by an anti-interleukin 2 receptor β chain monoclonal antibody in mice." The Journal of Experimental Medicine. 178. 1103-1107 (1993)
Description
「研究成果報告書概要(和文)」より
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[Publications] Kondo,A.,Imada,K.,Hattori,T.,Yamabe,H.,Tanaka,T.,Miyasaka,M.,Okuma,M.and Uchiyama,T.: "A model of in vivo cell proliferation of adult T-cell leukemia." Blood. 82. 2501-2509 (1993)
Description
「研究成果報告書概要(和文)」より
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[Publications] Tanaka, T., Kitamura, F., Nagasaka, Y., Kuida, K., Suwa, H.and Miyasaka, M.: "Selective long-term elimination of natural killer cells in vivo by an anti-interleukin 2 receptor beta chain monoclonal antibody in mice." J.Exp.Med.178. 1103-1107 (1993)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kondo, A., Imada, K., Hattori, T., Yamabe, H., Tanaka, T., Miyasaka, M., Okuma, M.and Uchiyama, T.: "A model of in vivo cell proliferation of adult T-cell leukemia." Blood. 82. 2501-2509 (1993)
Description
「研究成果報告書概要(欧文)」より
