1993 Fiscal Year Final Research Report Summary
DETECTION AND EVALUATION OF INTERACTION IN SOLUTION WITH SECONDARY EQUILIBRIUM LIQUID CHROMATOGRAPHY
| Project/Area Number |
04640547
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
分析・地球化学
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| Research Institution | Shizuoka University |
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Principal Investigator |
OKADA Tetsuo SHIZUOKA UNIVERSITY, FACULTY OF LIBERAL ARTS, ASSOCIATE PROFESSOR, 教養部, 助教授 (20183030)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Liquid chromatography / micelles / partition / polyethers / complexation |
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| Research Abstract |
Results obtained in the present research can be summarized as follows :
1. The partition of ions into an oppositely charged ionic micelle can be described by an electrostatic model or conveniently by an ion-exchange model. The partition of transition metal cations into an anionic micelles can be for example represented by the model involving the concentrations of a counter ion and a ligand. Secondary equilibrium liquid chromatographic investigation has indicated that the complexation of a metal ion at the interface between micelles and solution is suppressed by the surface charges of the micelles. This method has also permitted the evaluation of the dissociation of a micelle, when applied to the separation of anions with a cationic micelle.
2. Secondary equilibrium chromatography has allowed us to determine the complexation constants of crown-ethers with some hard cations. I have not found no marked difference in the complexation between in solution phase and in the ion-exchange resin phase. This fact has been utilized to develop a novel separation method of crown-ethers and to optimize the experimental condition for precise determination of the complexation constants.
3. It has been found that the partition of polyoxyethylenes is affected by the conformational changes of polyoxyethylenes. Differences in energy between stable conformers (helix and zigzag form) have been calculated by molecular mechanics. The results have been involved in a retention model of polyoxyethylenes. The analysis based on the developed model has well explained the retention behaviors.
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