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1993 Fiscal Year Final Research Report Summary

Neural and hormonal control mechanisms in excitatory cardiac regulation of the hermit crabs

Research Project

Project/Area Number 04640666
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionTokyo Metropolitan University

Principal Investigator

YAZAWA Tohru  Tokyo Metropolitan University Department of Biology, Joshu, 理学部, 助手 (30106603)

Co-Investigator(Kenkyū-buntansha) KUWASAWA Kiyoaki  Tokyo Metropolitan University Department of Biology, Professor, 理学部, 教授 (10015589)
Project Period (FY) 1992 – 1993
KeywordsDecapod Crustaceans / Heart / Proctolin / TTX / cAMP / Pacemaker Neuron / Driver Potential / Calcium Ions
Research Abstract

We studied the effects, on the A.aniculus heart, of a crustacean pericardial peptide, proctolin. Those effects were investigated by means of recording contractile force, intracellular membrane potential of the myocardium and extracellular potential from cardiac ganglionic cells. We used semi-intact hearts, isolated together with the dorsal, carapace, as well as totally isolated hearts. Threshold concentrations for positively inotropic and positively tonotropic effects were approximately 10^<-13> M and 10^<-10> M, respectively. At 10^<-7> M, inotropic effects amounted to more than 150% of the normal beat amplitude. Initially positive chronotropic effects were followed by negative effects during a period of perfusion with proctolin (10^<-6> M - 10^<-5> M). The inotropic effects resulted mainly from augmentation of the intra-burst impulse rate of cardiac ganglionic cells. The positively chronotropic effects (<50%) reflected increases in burst rate of the ganglion. The amplitude of the compound EJPs and that of the muscle action potentials were augmented, while conductance of the myocardial cell membrane was not changed, during proctolin application. Positively tonotropic effects were observed as contracture even in TTX-containing saline. The contracture was abolished in saline containing a protein kinase inhibitor, H-7. H-7 did not affect myocardial membrane potential but slightly enhanced ganglionic activity. An adenylate cyclase activator, forskolin, and a G-protein activator, cholera toxin, enhanced ganglionic activity but not contracture. In the presence of A1F_4^-, proctolin effects were not augmented. Certain phosphodiesterase inhibitors, caffeine, theophylline, 3-methylxanthine and IBMX affected the ganglion but not myocardial activity itself. That is, in the presence of the inhibitors, proctolin actions on the cardiac ganglion, but not on the myocardial cells, were significantly augmented. Not dibutyryl cGMP, but instead

  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] T.Yazawa: "Dopaminergic acceleration and GABAergic inhibition in extrinsic neural control of the hermit crab heart." Journal of Comparative Physiology. 174. 65-75 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yazawa: "Intrinsic and extrinsic neural and neurohumoral control of the decapod heart." Experientia. 48. 834-840 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yazawa: "Cholinergic small cells and dopaminergic large cells in the cardiac ganglion of hermit crabs.(投稿中)"

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yazawa and K.Kuwasawa: "Dopaminergic acceleration and GABAergic inhibition in extrinsic neural control of the hermit crab heart." J.Comparative Physiology A. 174-1. 65-75 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Yazawa and K.Kuwasawa: "Intrinsic and extrinsic neural and neurohumoral control of the decapod heart." Experientia. 48. 834-840 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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