1993 Fiscal Year Final Research Report Summary
Phylogenetic analysis of dinoflagellates using the birefringent nature of their chromosomes : why histone has been deleted from their chromosome structure?
| Project/Area Number |
04640676
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物生理・代謝
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| Research Institution | Nagano University |
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Principal Investigator |
SATO Hidemi Nagano University, Depertment of industry and Socuak Scuebce, Professor, 産業社会学部, 教授 (40109260)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Koichi Nagoya City University, College of General Education Professor, 教養部, 教授 (30080287)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Dinoflagellates / Histone / Chromosomal structure / Birefringence / Biochmistry |
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| Research Abstract |
Dinoflagellates can be classified into three groups following their birefringent nature of chromosomes in both resting and dividing stages. However, due to the lack of histone from their nuclei, it is widely supported that there is no sign of the existing of nucleosome. In fact, we proved the presence of nucleosome-like structure in wide variety of dinoflagellates. Using the mass cultured Oxyrrhis marina, a representative dinoflagellate isolated by Kato, we attempted to analyze the biochemical nature of the basic protein which substituting histone in the nuclei of dinoflagellates. Isolated neclear fraction of Oxyrrhis was processed following the standardized method of histone extraction. Protein components were analyzed on the SDS-PAGE.By comparine with histones obtained from both sea urchin embryo and calf thymus, we confirmed this basic protein is totally new and rather widely distributes in dinoflagellates nuclei clearly take over the role of histone. MW of this protein is 23 kD.Monoclonal antibody production is in progress. Their biochemical characteristics, function (s) and spatial distoribution of this protein were examined and reported in the text.
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[Publications] Soyer, M.O., Geraud, M.L., Coulaud, D., Barray, M., Theveny, B., Revet, B., Delan, M.: "Location of B- and Z-DNA in the chromosomes of primitive eukaryote dinoflagellates." J.Cell Biol.111 ;. 293-308 (1990)
Description
「研究成果報告書概要(欧文)」より
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