1993 Fiscal Year Final Research Report Summary
Regulation of fertilization and pollen selection in rice
| Project/Area Number |
04660001
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | HOKAIDO UNIVERSITY |
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Principal Investigator |
MAEKAWA Masashiko Hokkaido University, Dep.Agr., Associate Professor, 農学部, 助教授 (00142703)
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| Co-Investigator(Kenkyū-buntansha) |
INUKAI Tsuyoshi Hokkaido University, Dep.Agr., Instructor, 農学部, 助手 (90223239)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Pollen / Gametophyte gene / Fertilization / Pollen tube / Pollination |
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| Research Abstract |
The results obtained in the sutdy are as follows ;
(1) Function of gametophyte gene
Function of gametophyte gene, g^<a-6> was inverstigated by using the isogeneic line, T-65 g^<a-6> which possesses g^<a-6> in T-65.
In the same-timing pollination of T-65 and T-65 g^<a-6>, pollens with g^<a-6> show fertilization rate of about 1/3 compared with normal pollens. In the time-lagged pollination of pollens with g^<a-6>, lately pollinated pollens with g^<a-6> show fertilization rate of below 1/4 compared with formerly pollinated normal pollens. This result suggests that thers is differential elongation of pollen tubes getween g^<a-6> and normal pollens.
(2) Elongation of pollen tube with g^<a-6>
Germination tests of pollens in T-65, T-65 ^1g and T-65 g^<a-6> do not show any differences of germination rates in 6 minutes from shedding pollens on artificial medium. Elongation of pollen tubes in T-65 g^<a-6> are the shortest in the all in 6 minutes from shedding pollens. In 12 minutes, T-65 and T-65 ^1g stop elongation of pollen tubes and show shorter elongation than T-65 g^<a-6>. Although any clear differences of pollen tube elongation among T-65, T-65 ^1g or T-65 g^<a-6> are not recognized, it is presumed that g^<a-6> influence elongation of pollen tube.
(3) Temperature effect of fertilization of g^<a-6>
Crossing experiment, T-65/(T-65 g^<a-6>/T-65), in 28ﾟC show inceased ferquency of ^1g than in 20ﾟC.However, the frequencies of ^1g are variated. This result suggests that fertilization of g^<a-6> is influenced by temperature.
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