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1993 Fiscal Year Final Research Report Summary

Evolutionary relationship of biphenyl-catabolic genes in soil bacteria

Research Project

Project/Area Number 04660124
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・発酵学
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

FURUKAWA Kensuke  Kyushu University, Department of Argricultural Chemistry, Associate Professor, 農学部, 助教授 (90221556)

Co-Investigator(Kenkyū-buntansha) YOSHINO Sadazou  Kyushu University, Department of Argricultural Chemistry, Assitant Professor, 農学部, 助手 (80117291)
Project Period (FY) 1992 – 1993
KeywordsBiphenyl-utilizing bacteria / Toluene-utilizing bacteria / Catabolic genes / Nucleotide sequence / Molecular evolution / Genetic homology / Dioxygenase / Substrate specificity
Research Abstract

Biphenyl(BP) utilizing bacteria are widely distributed in the environment. Thirty two different BP-strains isolated far clasified into four groups according to the homologies of BP-catabolic bph genes and immunological properties of BP-ring-cleavage dioxygenases. The bph operon of Pseudomonas pseudoalcaligenes KF707 is organized as bphA1A2A3A4BCXD.Biphenyl dioxygenase is a multi-component enzyme which composed of large subunit(encoded by bphA1) and small subunit(encoded by bphA2)of terminal deioxygenase, ferredoxin(encoded by bphA3)and ferredoxin reductase(encoded by bphA4). bphB encodes BP-dihydrodiol dehydrogenase, bphC encodes BP-ring meta-cleavage dioxygenase, and bphD encodes hydrolase. The bph operon is very similar to toluene catabolic tod operon of Pseudomonas putida F1 in the gene organization and nucleotide sequences. The gene components responsible for substrate specificity between the bph and tod operons were investigated. The large and small subunits of the terminal dioxygease and the ring meta-cleavage compound hydrolase were critical for their discrete metabolic specificities. From the results obtained in this study it can be postulated that BP-utilizing bacteria, along with other aromatics utilizing bacteira, might be involved in the final degradation of plant lignin.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Furukawa,K.et al.: "Biochemical and genetic basis for the degrad-ation of polychlorinated biphenyls in soil bacteria" Pseudomonas:Molecular Biology and Biotechnology. 259-267 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furukawa,K.: "Gene coding for PCB-degradation in soil bacteria" Now and Future. 8. 13-16 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furukawa,K.et al.: "Gene components responsible for discrete substrate specificity in the catabolism of biphenyl(bph operon)and toluene(tod operon)" J.Bacteriol.175. 5224-5232 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furukawa,K.et al.: "Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase" J.Bacteriol.(印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furukawa,K.et al.: "Construction of hybrid biphenyl(bph)and toluene(tod)genes for functional analysis of aromatic ring dioxygenases" Gene. 138. 27-33 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furukawa,K.: "Molecular genetics and evolutionary relation-ship of PCB-degrading bacteria" Biodegradation. (印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Furukawa, S.Hayashida and K.Taira: "Biochemical and genetic basis for the degradation of polychlorinated biphenyls in soil bacteria" Pseudomonas : Molecular Biology and Biotechnology. 259-267 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Furukawa: "Genes coding for PCB-degradation in soil bacteria" Now and Future. 8. 13-16 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Furukawa, J.Hirose, A.Suyama, A.Zaili and S.Hayashida: "Gene components responsible for discrete substrate specificity in the catabolism of biphenyl (bph operon) and toluene (tod operon)" J.Bacteriol. 175. 5224-5232 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Furukawa, J.Hirose, S.Hayashida and K.Nakamura: "Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase" J.Bacteriol. 176(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Hirose, A.Suyama, S.Hayashida and K.Fukrukawa: "Construction of hybrid biphenyl (bph) and toluene (tod) genes for functional analysis of aromatic ring dioxygenases" Gene. 138. 27-33 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Furukawa: "Molecular genetics and evolutionary relationship of PCB-degrading bacteria" Biodegradation. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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