1993 Fiscal Year Final Research Report Summary
Study on the role of epidermal growth factor receptor and protooncogenes in mammalian morphogenesis
| Project/Area Number |
04670014
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SHIOTA Kohei Kyoto Univ.Faculty of Medicine, Department of Anatomy, Professor, 医学部, 教授 (80109529)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIBASHI Makoto Kyoto Univ.Faculty of Medicine, Department of Anatomy, Instructor, 医学部, 助手 (30232341)
MORI Chisato Kyoto Univ.Faculty of Medicine Department of Anatomy, Assoc.Prof, 医学部, 助教授 (90174375)
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| Project Period (FY) |
1992 – 1993
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| Keywords | morphogenesis / EGF receptor / proto-oncogene / palatogenesis / birth defect / proliferation / differentiation |
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| Research Abstract |
1. Expression and localization of epidermal growth factor receptor (EGF-R)in human embryonic tissues
Human embryos of 3-8 weeks of gestation were immersion-fixed in 4% paraformaldehyde, embedded in paraffin, and serially sectioned. They were immunostained with anti-rabbit EGF-R antibody and examined after diaminobenzidine/H_2O_2 reaction. Characteristic tissue-specific localization of EGF-R was noted, and the epithelia of the digestive tube and respiratory tract, cartilage, kidney, and epidermis were strongly positive for the reactive products against EGF-R.Proliferating epithelial tissues were selectively reactive, suggesting that epidermal growth factor may play a key role in the proliferation and differentiation of epithelial tissues in human embryos.
2. Expression of EGF-R in the fusing palatal shelves of mouse fetuses.
Expression of EGF-R was examined immunohistochemically in the palates of days-12-14 mouse fetuses. Immunoreactive products against EGF-R was detected in palatal epithelia as well as underlying mesenchyme, and spatial heterogeneity and temporal sequence were noted which arppeared closely related to the palatogenetic process.
3. Expression of EGF-R in dysmorphogenesis
Cleft Palate is frequently produced if pregnant female mice are treated with retinoic acid. Pregnant female mice were given orally 80-100 mg/kg all-trans retinoic acid and their fetuses were examined immurnohistochemically for EGF-R.The expression of EGF-R in the palatal tissue of treated fetuses were significantly more intense than in the corresponding tissue of control fetuses. It seems that retinoic acid disturbs the normal regulation of EGF-R expression and thereby inhibit the fusion of palatal shelves.
The present study has shown that epidermal growth factor and related protooncogenes may play important role in mammalian morphogenesis.
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[Publications] Minote, H., Inoue, K., Higashide, S., Tobe, T., Takeuchi, E., Mori, C., and Shiota, K.: "Immunohistochemical study on epidermal growth factor receptor during carcinogenesis in the rat liver." Arch.Jpn.Chirurg. 61. 249-258 (1992)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Higashide, S., Inoue, K., Minote, H., Tobe, T., Mori, C.and shiota, K.: "Expression of epidermal growth factor receptor after partial pancreatectomy in adult rats : An immunohistochemical study." Arch, Jpn.Chirurg. 61. 311-319 (1992)
Description
「研究成果報告書概要(欧文)」より
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