1993 Fiscal Year Final Research Report Summary
Physiological functions of gangliosides and these applications
| Project/Area Number |
04670155
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
OSANAI Taka Tokyo Metropolitan Institute of Medical Science, Biochemical Cell Research, senior researcher, 生命情報研究部門, 研究員 (60126018)
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| Co-Investigator(Kenkyū-buntansha) |
AOYAGI Takaaki 昭和薬科大学, 衛生化学, 教授 (10159303)
NAGAI Yoshitaka (財)東京都臨床医学総合研究所, 所長 (80072974)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Gangliosides / Enzyme activities / Protesae inhibitors / Galactosidase / Proteases / GM2 deficiency / WHT / Ht mouse |
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| Research Abstract |
1).Dose-dependent enzyme suppression in spleen induced by GM1 administration to mice
Our previous studies suggested that the administration of exogenous gangliosides to the body modulates enzymatic networks in the brain. In the present study, we tested whether that is the case with another organ, spleen. By testing the dose response relationship, we found that there is a optimum dose for the effect of enzymatic modulation of GM1 administration. Although the optimum level varied depending on each of the examined hydrolytic enzymes, it usually fell in the range around 50 mug/kg body weight. The findings led us to conclude that the enzyme-modulating actions of gangliosides come not merely from the bizarre actions in vivo of high molecular exogegeous substances.(Chem.Pharm.Bull.'40,1958-1960,1992)
2).Deficiency of galactosidase activity in multiple organs in GM2-deficient mice
WHT/Ht mice are known to have GM2 deficiency especially in the liver. In order to known the relationship of this abnormality to the general metabolism in organs or cells, we compared 17 hydrolytic enzyme activities in the liver, brain, spleen, and kidney between the WHT/Ht mice and control BALB/c mice or without administration of GM2 and GM3. Regardless of the GM2 or GM3 treatment, the galactosidase activity was markedly low in all of the tested organs in the WHT/Ht mice when compared with that in the control animals. This consistency stands in contrast with the known organ-dependency heterogeneity of ganglioside expression and thus indicates independency of the GM2 deficiency from the galactosidase deficiency in this model animal.(J.Clin.Biochem.Nutr., 13,189-197,1992)
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