1992 Fiscal Year Annual Research Report

潰瘍性大腸炎一大腸癌モデルに関する実験病理学的研究

Research Project

Project/Area Number	04670202
Research Institution	Gifu University
Principal Investigator	森秀樹岐阜大学, 医学部, 教授 (70021433)
Co-Investigator(Kenkyū-buntansha)	杉江茂幸岐阜大学, 医学部・附属病院, 助手 (60187648) 吉見直己岐阜大学, 医学部, 助手 (30166996) 田中卓二岐阜大学, 医学部, 助教授 (40126743)
Keywords	Hydroxyanthraquinone / BrdU / 大腸粘膜 / ODC活性 / 陰高長 / 潰瘍性病変 / メラノーシス / ラット
Research Abstract	1-HydroxyanthraquinoneをACIラットに1%の濃度にて飼料投与し、投与開始後4、8、12ヶ月にて屠殺し、盲腸、上部結腸、下部結腸粘膜上皮の細胞増殖性を粘膜上皮の厚さ、BrdUの標識率、ornithine decarboxylase(ODC)活性の測定によりassayし、又潰瘍性病変、陰高膿瘍、メラノーシス等の変化を経時的に検索した。陰高長は経過と共に延長し、その傾向は盲腸、上部結腸に於いて強かった。BrdUの標識率では陰高長と類似せる傾向を示し、1-hydroxyanthraquinoneの投与を受けたラットでは対照群に比し、4、8、12ヶ月のいずれの時点でも高い標識率を示した。とくに12ヶ月時ではその傾向が顕著であった。ODC活性は盲腸、上部結腸、下部結腸のいずれに於いても4、8、12ヶ月時共1-hydroxyanthraquinoneの投与ラットが対照ラットよりも有意に高かった。メラノーシスの出現は盲腸では4ヶ月よりみられ、結腸では8ヶ月より認められた。陰高膿瘍の出現は盲腸では8ヶ月よりみられ、結腸では12ヶ月より認められた。潰瘍の形成は盲腸では8ヶ月よりみられ、結腸では12ヶ月より認められ、一般にその程度は盲腸に於いて強かった。以上の1-hydroxyanthraquinone投与ラットに於ける大腸粘膜上皮の経時的観察により、腫瘍発生部位に一致して粘膜上皮細胞の増殖性の亢進があることが分り、細胞増殖性のhydroxyanthraquinone発癌に於ける重要な意義が明らかにされた。又、潰瘍性病変の出現部位と腫瘍発生部位もほぼ一致し、同病変の出現と発癌との密接な関係も示唆された。

Research Products

(8 results)

All Other

All Publications (8 results)

[Publications] Mori,H., Morishita,Y., Mori,Y., Yoshimi,N., Sugie,S., Tanaka,T.: "Effect of magnesium hydroxide on methylazoxymethanol-induced epithelial proliferation in the large bowels of rat" Cancer Letters. 61. 43-89 (1992)
[Publications] Mori,H., Morishita,Y., Tanaka,T., Okumu-ra,A., Wang,A.: "Effect of magensium hydroxide on the cell cycle of muco-sal epithelium; A mechanism of the suppressive action onthe carcinogen-induced large bowel carcinogenesis in rats" Journal of Japanese Society of Magnesium Research. 11. 93-101 (1992)
[Publications] Mori,H., Morishita,Y., Sugie,S., Tanaka,T., Kojima,T., Suzui,M.: "Carcinogenicity of examination of 1-nitropyrene oxides and related chemicals; Lack of their tumorigenic effects in a newborn mice assay" Journal of Toxicological Sciences. 17. 235-241 (1992)
[Publications] Mori,H., Yoshimi,N., Sugie,S., Tanaka,T., Morshita,Y., Jinlog: "Inhibitory effect of 5-hydroxy-4-(2-phenyl-(E)-ethenyl)-2(5H)-furanone, a novel synthesized retinoid on azoxy-methane-induced intestinal carcinogenesis in rats" Cancer Letters. 66. 93-98 (1992)
[Publications] Mori,H., Mori,Y., Tanaka,T., Yoshimi,N., Sugie,S., Kawamori: "Cell kinetic analysis of the mucosal epithelium and ass-ay of ornithine decarboxylase activity during the pro-cess of 1-hydroxyanthraquinone-induced large bowel carci" Carcinogenesis. 13. (1992)
[Publications] Sugie,S., Yoshimi,N., Okumura,A., Tanaka,T., Mori,H.: "Modifying effects of benzyl isothiocyanate on DNA synthe-sis in primary culture of rat hepatocytes: a possible assay for chemopreventive agents" Carcinogenesis. 14. (1993)
[Publications] Mori,H.: "Genotoxicity of naturally occurring metabolites; Structu-ral analogs of aflatoxin and related chemicals in″Applied Mycology 5; eds by D. Bhatnager et al.″" Marcel Dekker Inc., New York/Basel, 231-253 (1992)
[Publications] Mori,H., Morishita,Y., Shinoda,T., Tanaka,T.: "Preventive effect of magnesium hydroxide on carcinogen-induced large bowel carcinogenesis in rats. in″Antimuta-genesis and Anticarcinogenesis Mechanism III″" Prenum Press, (1993)

1992 Fiscal Year Annual Research Report

潰瘍性大腸炎一大腸癌モデルに関する実験病理学的研究

Principal Investigator

森 秀樹 岐阜大学, 医学部, 教授 (70021433)

Research Products

[Publications] Mori,H., Morishita,Y., Mori,Y., Yoshimi,N., Sugie,S., Tanaka,T.: "Effect of magnesium hydroxide on methylazoxymethanol-induced epithelial proliferation in the large bowels of rat" Cancer Letters. 61. 43-89 (1992)

[Publications] Mori,H., Morishita,Y., Sugie,S., Tanaka,T., Kojima,T., Suzui,M.: "Carcinogenicity of examination of 1-nitropyrene oxides and related chemicals; Lack of their tumorigenic effects in a newborn mice assay" Journal of Toxicological Sciences. 17. 235-241 (1992)

[Publications] Mori,H., Yoshimi,N., Sugie,S., Tanaka,T., Morshita,Y., Jinlog: "Inhibitory effect of 5-hydroxy-4-(2-phenyl-(E)-ethenyl)-2(5H)-furanone, a novel synthesized retinoid on azoxy-methane-induced intestinal carcinogenesis in rats" Cancer Letters. 66. 93-98 (1992)

[Publications] Mori,H., Mori,Y., Tanaka,T., Yoshimi,N., Sugie,S., Kawamori: "Cell kinetic analysis of the mucosal epithelium and ass-ay of ornithine decarboxylase activity during the pro-cess of 1-hydroxyanthraquinone-induced large bowel carci" Carcinogenesis. 13. (1992)

[Publications] Sugie,S., Yoshimi,N., Okumura,A., Tanaka,T., Mori,H.: "Modifying effects of benzyl isothiocyanate on DNA synthe-sis in primary culture of rat hepatocytes: a possible assay for chemopreventive agents" Carcinogenesis. 14. (1993)

[Publications] Mori,H.: "Genotoxicity of naturally occurring metabolites; Structu-ral analogs of aflatoxin and related chemicals in″Applied Mycology 5; eds by D. Bhatnager et al.″" Marcel Dekker Inc., New York/Basel, 231-253 (1992)

[Publications] Mori,H., Morishita,Y., Shinoda,T., Tanaka,T.: "Preventive effect of magnesium hydroxide on carcinogen-induced large bowel carcinogenesis in rats. in″Antimuta-genesis and Anticarcinogenesis Mechanism III″" Prenum Press, (1993)

森秀樹岐阜大学, 医学部, 教授 (70021433)