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1993 Fiscal Year Final Research Report Summary

Studies on the Bacterial Stress Proteins as Virulence Factors

Research Project

Project/Area Number 04670252
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionKyorin University

Principal Investigator

YAMAMOTO T.  Kyorin Univ. Medicine, Associate Professor, 医学部, 助教授 (60110342)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI H.  Kyorin Univ. Medidine, Research Assistant, 医学部, 助手 (40221650)
TAGUCHI H.  Kyorin Univ. Medicine, Research Assistant, 医学部, 助手 (20146541)
OGATA S  Kyorin Univ. Medicine, Professor, 医学部, 教授 (40086533)
Project Period (FY) 1992 – 1993
KeywordsSTRESS PROTEIN / VIRURENCE FACTOR / Yersinia enterocolitica / マクロファージ
Research Abstract

Yersinia enterrocolitica is a facultative intracellular pathogen capable of surviving within professional phagocytic cells. Since the internal environment is stressful for the phagocytosed bacteria, the bateria responds by induction of stress proteins which possibly contribute to intracellular survival. To identify the stress-responding genes important for intracellular survival, a total 1800 mutants of Y.enterrocolitica with independent Tn10-insertion were screened for the stress-sennsitive mutations that allow normal growth at 30゚C but either block or result in very poor bacterial growth at high temperature, 39゚C.The resulting 42 mutants with thermosensitive phenotype for vacterial growth were then screened for the mutation with a diminished capacity for intracellular survival using an in vitro asay for survival in macrophagges. A mutant named Gts 154 unable to survive within macrophages was less virulent than the wild-type parent in vivo. Theresult suggest the stress-responding gene at the stress envitonment in vitro involves in the survival and growth in the macrophage which is an imoportant aspect of virulence of Y.enterocolitica. We then studied a coordinate regulation in gene expression of Y.enterrocolitica which possibly contributes to bacterial survival within phagocytes. At least 16 proteins were selectively induced in response to phagocytosis. Several macrophage-induced proteins were also induced by heat shock and oxidative stresses in vitro. Of those, two major stress proteins, DnaK and CRPA, were immunologically identified. These results indicated that intracellular Y.enterocolitica exhibits a global stress respponse to the hostille environment of the macrophage.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] D.Wall,J.M.Delaney,O.Fayet,B.Lipinska,T.Yamamoto and C.Georgopoulos: "arc-dependent thermal regulation and extragenic suppression of the Escherichia coli cytochrome d operon" Journal of Bacteriology. 174. 6554-6562 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Yamaguchi,T.Yamamoto,Y.Konoeda,H.Taguchi and S.Ogata: "Epitope homology between bacterial heat shock protein and self-proteins in the host cell" APMIS. 100. 957-962 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yamamoto,H.Miura,K.Ohsumi,H.Yamaguchi,H.Taguchi and S.Ogata: "Cloning and nucleotide sequence analysis of immunodominant heat-shock protein of Yersinia enterocolitica" Research in Microbiology. 144. 691-701 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yamamoto,T.Hanawa,and S.Ogata: "Induction of Yersinia enterocolitica stress proteins by phagocytosis with macrophages" Microbiology and Immunology. 38(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] D.Wall, J.M.Delaney, O.Fayt, B.Lipinska, T.yamamoto and C.Georgopoulos: "arc-dependent thermal regulation and extragenic suppression of the Escherichia coli cytochrome d operon." J.Bacteriol. 174. 6554-6562 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Yamaguchi, T.Yamamoto, Y.Konoeda, H.Taguchi and S.ogata: "Epitope homology between bacterial heat shock protein and self-protein in the host cell." APMIS. 100. 957-962 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Yamamoto, H.Miura, K.Ohsumi, H.Yamaguchi, H.Taguchi and S.Ogata: "Cloning and nucleotide sequence analysis of immunodominant heat-shock protein of Yershinia enterocolitica." Res. Microbiol.144. 691-701 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Yamamoto, T.Hanawa, and S.Ogata: "Induction of Yershinia enterrocolitica proteins by phagocytosis with macrophages." Microbiol. Immunol. 38 (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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