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1993 Fiscal Year Final Research Report Summary

Identification of Determinants for Tropism of Sendai virus

Research Project

Project/Area Number 04670278
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionJichi Medical School

Principal Investigator

TASHIRO Masato  Jichi Medical School, Dept.Virol., Assoc.Prof., 医学部, 助教授 (90111343)

Co-Investigator(Kenkyū-buntansha) ODAGIRI Takato  Jichi Medical School, Dept.Virol., Assis.Prof., 医学部, 講師 (80177237)
TANAKA Toshinori  Jichi Medical School, Dept.Virol., Assis.Prof., 医学部, 講師 (30146154)
Project Period (FY) 1992 – 1993
KeywordsSendai virus / Tropism / Tryptase Clara / Budding polarity / Mictotubules / M protein / Epithelial cells / Protein transport
Research Abstract

1. We isolated and characterized a novel serine-protease designated "Tryptase Clara", present in and secreted from the Clara cells of rat bronchial epithelia. Tryptase Clara was revealed to activate Sendai virus progeny by proteolytic cleavage of the F protein in rat lungs, and thereby facilitate multiple cycles of viral replication and pneumopathology.
2. Budding polarity of Sendai virus at the bronchial epithelial cells, the primary target of infection, was shown to determine the organ tropism. Apical budding by wild-type virus is responsible for licalized infection in the airways, whereas basolateral budding is required for systemic spread of progeny virus to distant organs resulting in a systemic infection.
3. In addition to proteolytic activation of the F protein, basolateral expression of viral glycoproteins is a prerequisite for inducing cell fusion.
4. A mutant Sendai virus, F1-R, which buds bipolarly in epithelial cells, was shown to disrupt microtubule network in infected cells, resulting in impaired cellular polarity. By comparative analysis of the entire nucleotide sequence of wild-type and F1-R viruses, the mutated M protein was suggested to be responsible for this phenomenon.

  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] M.Tashiro: "Budding site of Sendai virus in polarized epithelial cells is one of the determinants for tropism and pathogenicity in mice." Virology. 187. 413-422 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Tashiro: "Changes in specific cleavability of the Sendai virus F protein:implications for pathogenicity in mice." J.Gen.Virol.73. 1575-1579 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Tashiro: "Significance of basolateral domain of polarized MDCK cells for Sendai virus-induced cell fusion." Arch.Virol.125. 129-139 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Tashiro: "Tryptase Clara,an activating protease for Sendai virus in rat lungs,is involved in pneumopathogenicity." J.Virol.66. 7211-7216 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Kido: "Isolation and characterization of a novel trypsin-like protease found in rat bronchiolar epithelial Clara cells:a possible activator of the viral fusion glycoprotein." J.Biol.Chem.267. 13573-13579 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Scheiblauer: "Interactions between bacteria and influenza A virus in the development of influenza pneumonia." J.Infect.Dis.166. 783-791 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Tashiro: "Antibody against the carboxyl terminus of the F2 subunit of Sendai virus fusion glycoprotein inhibits proteolytic activation." Virology. 194. 882-885 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Tashiro: "Possible involvement of microtubule disruption in bipolar budding of a Sendai virus mutant,F1-R,in epithelial MDCK cells." J.Virol.67. 5902-5910 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Urabe: "Persistence of viral genes in a variant of MDCK cells after productive infection with a mutant of influenza virus A/WSN." Arch.Virol.128. 97-110 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Kido: "Pulmonary surfactant is a potential inhibitor of proteolytic activation of Sendai virus and influenza A virus." FEBS lett.322. 115-119 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Sakai: "Sendai virus infection changes the subcellular localization of tryptase Clara,an activating protease for pneumotropic viruses,in rat bronchiolar epithelial cells." Eur.Respi.J.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田代真人: "パラミクソウイルスの病原性発現機構と生体防御" 生体防御. 9. 155-164 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田代真人: "MDCK細胞 瀬野、小山、黒木(編)研究テーマ別動物培養細胞マニュアル" 共立出版, (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Homma: "Sendai virus.R.G.Webster & A.Garanoff(eds)Encyclopedia of Virology" Academic press,London(in press), (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Tashiro, J.T.Seto, S.Choosakul, M.Yamakawa, H.-D.Klenk, R.Rott: "Budding site of Sendai virus in polarized epithelial cells is one of the determinants for tropism and pathogenicity in mice." Virology. 187(2). 413-422 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Tashiro, J.T.Seto, S.Choosakul, H.Hegemann, H.-D.Klenk, R.Rott: "Changes in specific cleavability of the Sendai virus F protein : implications for pathogenicity in mice." J.Gen.Virol.73(6). 1575-1579 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Tashiro, M.Yamakawa, K.Tobita, J.T.Seto, H.-D.Klenk, R.Rott: "Significance of basolateral domain of polarized MDCK cells for Sendai virus-induced cell fusion." Arch.Virol.125(1-4). 129-139 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Tashiro, Y.Yokogoshi, K.Tobita, J.T.Seto, R.Rott, H.Kido: "Tryptase Clara, an activating protease for Sendai virus in rat lungs, is involved in pneumo-pathogenicity." J.Virol.66(12). 7211-7216 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Kido, Y.Yokogoshi, K.Sakai, M.Tashiro, Y.Kishino, A.Fukutomi, N.Katunuma: "Isolation and characterization of a novel trypsin-like protease found in rat bronchiolar epithelial Clara cells : a possible activator of the viral fusion glycoprotein." J.Biol.Chem.267(19). 13573-13579 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Scheiblauer, M.Reinacher, M.Tashiro, R.Rott: "Interactions between bacteria and influenza A virus in the development of influenza pneumonia." J.Infect.Dis.166(4). 783-791 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Tashiro, M.Takeda, S.Tanaka, N.Nishimura, M.Takenaka, H.Kido: "Antibody against the carboxyl terminus of the F2 subunit of Sendai virus fusion glycoprotein inhibits proteolytic activation." Virology. 194(2). 882-885 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Tashiro, J.T.Seto, H.-D.Klenk, R.Rott: "Possible involvement of microtubule disruption in bipolar budding of a Sendai virus mutant, F1-R, in epithelial MDCK cells." J.Virol.67(10). 5902-5910 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Urabe, T.Tanaka, T.Odagiri, M.Tashiro, K.Tobita: "Persistence of viral genes in a variant of MDCK cells after productive infection with a mutant of influenza virus A/WSN." Arch.Virol.128(1-4). 97-110 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Kido, K.Sakai, Y.Kishino, M.Tashiro: "Pulmonary surfactant is a potential inhibitor of proteolytic activation of Sendai virus and influenza A virus." FEBS lett.322(2). 115-119 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Sakai, T.Kohri, M.Tashiro, Y.Kishino, H.Kido: "Sendai virus infection changes the subcellular localization of tryptase Clara, an activating protease for pneumotropic viruses, in rat bronchiolar epithelial cells." Eur.Respi.J.(in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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