1993 Fiscal Year Final Research Report Summary
A Growth Inhibitor of Rat Hepatocytes Synthesized by a Rat Hepatoma Cell Line, FF101
| Project/Area Number |
04670424
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
KAWASAKI Tsunehisa Hamamatsu University School of Medicine, The Second Department of Internal Medicine, Research Assistant, 医学部・第2内科, 助手 (80214625)
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| Co-Investigator(Kenkyū-buntansha) |
NEMOTO Masaki Hamamatsu University School of Medicine, The Second Department of Internal Medic, 医学部・第2内科, 医員
KOBAYASHI Yoshimasa Hamamatsu University School of Medicine, The Second Department of Internal Medic, 医学部・第2内科, 助手 (50252185)
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| Project Period (FY) |
1992 – 1993
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| Keywords | hepatocytes primary culture / growth factor / growth inhibitor / hepatoma / serum free culture |
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| Research Abstract |
A rat hepatoma cell line, FF101, established in serum-free, protein-free medium synthesizes a growth inhibitor of adult rat hepatocytes in primary culture. Gel filtration of FF101-conditioned medium disclosed two peaks of growth inhibitory activity with the molecular sizes over 200kD and smaller than 10kD.The growth inhibitor of smaller molecular size was tentatively designated as FF-GI.The DNA synthesis of rat hepatocytes was inhibited by FF-GI.The inhibitory activity of FF-GI was abolished by treatments with trypsin, dithiothreitol and heating at 100ﾟC for 30min, although it was stable against acid treatment and heating at 100ﾟC for 5min.
These results indicate that FF-GI may be a novel growth inhibitor of adult rat hepatocytes.
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