1993 Fiscal Year Final Research Report Summary
The Roles of Adhesion Molecules on the Mechanism of Hepatic disorder in Chronic Hepatits
| Project/Area Number |
04670426
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Nagoya University |
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Principal Investigator |
KOYAMA Yasuo Nagoya University, School of Medicine, Instructor, 医学部, 助手 (40192067)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUDA Yoshihide Nagoya Insitute of Techonology, Health adiministration Center, Asistant Professo, 保健管理センター, 講師 (40181284)
IMOTO Masami Nagoya University, School of Medicine, Asistant Professor, 医学部, 講師 (10158769)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Adhesion Molecule / Lymphoid follicle / Dendritic Cell / Chronic Hepatitis / Liver Cirrhosis / Hepatocellular Caricinoma / Immunohistochemistry |
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| Research Abstract |
Liver specimens with chronic hepatitis (CH), liver cirrhosis (LC) and hepatocellular cqrcinoma (HCC) were examined immunohistochemically. For the control study, normal liver specimens were also examined. Serum intercellular adhesion molecule-1 (ICAM-1) levels were determined by ELISA method among normal subjects, CH, LC and HCC.
In normal liver, expression of ICAM-1 and intercellular adhesion molecule-2 (ICAM-2) was restricted to sinusoidal lining cells and portal vascular endothelial cells. In CH and LC, ICAM-1 and ICAM-2 were enhanced in the area of necrosis. ICAM-1 was also expressed on hepatocytes in a honeycomb patern. Follicular dendritic cells in the center of lymphoid follicles were positive for ICAM-1. These cells were in contact with lymphocyte function-associated antigen-1. In HCC, ICAM-1 and ICAM-2 were detected in carcinomatous sinusoids and carcinoma cells. The capsule and sinusoids of HCC contained lymphocytes.
In contrast to controls, serum ICAM-1 levels were significantlly elevated in CH, LC and HCC.In the cases of CH, there was a significantcorrelation between the levels of ICAM-1 and ALT.The mean ICAM-1 level in HCC cases, no significant correlation was observed between ICAM-1 and tumor size or tumor markers (AFP and PIVKA-II).
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