• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1993 Fiscal Year Final Research Report Summary

The function of N-CAM in patients with neuromuscular diseases and axonal guidance.

Research Project

Project/Area Number 04670481
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Neurology
Research InstitutionAsahikawa Medical College

Principal Investigator

YAHARA Osamu  Asahikawa Medical College, Assistant, 医学部, 講師 (20133845)

Project Period (FY) 1992 – 1993
KeywordsN-CAM / in situ hybridization / neuromuscular diseases / embryo mice heart / 分化
Research Abstract

N-CAM is one of Immunoglobuin superfamily. We studied the function of N-CAM in patients with neuromuscular diseases and axonal guidance. There are four isoforms of N-CAM ; transmembrane form( spinal and cerebral form ), muscle specific form( MSD ), GPI form and secret form.
In patients with neuromuscular diseases, N-CAM had activity in the regenerating muscles of limb girdle dystrophy, myotonic dystrophy, Polymyositis and Kugelberg Welander. Immunoreactivity was shown strongly on muscles of Polymyositis, but no activity in muscles of motor neuron diseases. We thought that N-CAM may be a marker of regenerating muscles.
By using in situ hybridization technique with riboprobe, we studied the N-CAM expression of muscle and spinal cord in embryo mice. Transmenbrane form began to have signal in motor neuron of spinal cord on embryonic day 11. Until embryonic day 18, N-CAM had signals diffusely in the spinal cord. MSD form had expression in myotome on embryonic day 12, whereas GPI and secret forms had no signals in muscle and spinal cords. We demonstrated that N-CAM isoform were expressed temporal and spatial pattern in embryonic skeletal mus-cles. N-CAM may be important to developmental regulation of muscles.
We investigated that N-CAM expression was examined during cardiac development in the rat mouse using immunohistochemical technique. N-CAM appeared in embryonic day 12, then N-CAM had activity until about new born. After birth , decreased. Cardiac N-CAM expression is therefore subjects to temporal regulation and may modulate cellular interactivity in the developing heart.

  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] 南 宏明: "虚血による心筋調節タンパク質の変化" 医学のあゆみ. 165. 431-432 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 南 宏明: "老齢心における心機能低下の要因-二次元電気泳動法を用いての検討-" 日本老年医学会雑誌. 30. 617-621 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 南 宏明: "ストレプトゾトシン誘発糖尿病ラットにおける心筋ミオシン重鎖の減少" 医学のあゆみ. 167. 238-237 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osamu Yahara: "Immunoreactive manganese-superocxide dismutase in human serum under physical exercise neuromuscular disorder" Progress in Clinical Biochemi. 827-829 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Atsushi Obara: "Effect of xamoterol in Shy-Drager syndrome" Circulation. 85. 606-611 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinobu Osanai: "Charcot-Marie-Tooth disease with diaphragmatic weakness" Internal Medicine. 31. 1267-1270 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsuro Takeuchi: "Expression of NCAM isoforms during skeletal myogenesis in the mouse embryo" Developmental Dynamics. 194. 1-11 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1995-03-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi