1993 Fiscal Year Final Research Report Summary
Mechanism of rat astrocyte differentiation induced by glia maturation factor
| Project/Area Number |
04670495
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | Nagoya City University Medical Shcool |
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Principal Investigator |
ITO Jin-ichi Nagoya city University Medical School, Assistant Professor, 医学部, 講師 (60167260)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Ryo Nagoya city University Medical School, Assistant Professor, 医学部, 教授 (90094383)
TANAKA Ryo Nagoya city University Medical School, Assistant Professor (90094383)
TANAKA Ryo Nagoya city University Medical School, Assistant Professor (90094383)
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| Project Period (FY) |
1992 – 1993
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| Keywords | astrocyte / microfilament / glia filament / sialosylcholesterol |
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| Research Abstract |
We investigated the mechanism of action to glial cytoskeletons of glia maturation factor (GMF) that induced differntiation o fcultured rat astrocytes. Both depalymerised microfilament (MF) and depolymerized glia filament (GF) were separately extracted from the crude cytoskeletal fraction of rat astrocytes. The microfilament-like filaments reconstituted from MF by the addition of 100mM KCI and 10mM MgCl_2 contained not only actin, main component of microfilament, but also many minor components such as GFAP and vimentin, main component of glia filament. Purification of the reconstiruted filaments by S1 sepharose affinity column chromatography failed to remove glia filament components. The glia filament-like filaments reconstiruted from GF in the rpesence of 150 mM NaCl, 10 mM MgCl_2 were conposed of actin and other minor components in addition to vimentin and GFAP.
MF promoted GF polymerization in the presence of 150 mM NaCl, 10 mM MgCl_2 and alpha-sialosylcholesterol (SC), which suppressed polymerization both of MF and of GF.These findings suggested that MF promoted GF polymetization trough interaction between MF and GF.
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