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1994 Fiscal Year Final Research Report Summary

Research on molecular diagnosis of viral encephalitis in children -application to early diagnosis-

Research Project

Project/Area Number 04670589
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionNagoya University

Principal Investigator

MORISHIMA Tuneo  Nagoya University School of Medicine Assistant Profassor, 医学部, 講師 (90157892)

Co-Investigator(Kenkyū-buntansha) HORIBE Keizou  Nagoya University School of Medicine Assistant Profassor, 医学部, 講師 (30209308)
Project Period (FY) 1992 – 1994
KeywordsPCR assay / Herpes simplex virus / Herpes simplex encephalitis / Cytomegalovirus / intrauterine infection / DNA / encephalitis / encephalopathy
Research Abstract

(1) In herpes simplex encephalitis (HSE), detection of HSV DNA in CSF of patients during acute phase was performed by means of PCR assay. By PCR,the HSV DNA was found more than 90% of HSE patients, showing that this method is useful for early diagnosis.
(2) Quantiation of HSV DNA was performed and the mode of presense of viral genome was examined by PCR method. In neonatal HSE,large quantities of HSV DNA was found in CSF as a form of infectious particles, while in HSE of older Children, the amount of viral DNA was smaller and most of the viral DNA was fragmented.
(3) In West syndrome patients, who had been infected with human cytomegalovirus (CMV) transplacentally, viral DNA was detectable in CSF,suggesting that the presense of CMV in the brain could play some roles on the progress of West syndrome.
Thus' interesting reslults were obtained during these 3 years.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Osuga T et al.: "Transfer of specific IgG and IgGsubclasses to herpes simplex virus across the blood-brain barrier and placenta in preterm and term newborns." Acta Paediatr. 81. 792-796 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimura H et al.: "Relapse of herpes simplex encephalitis in children." Pediatr. 89. 891-894 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ando Y et al.: "Quantitative analysis of herpes simplex virus DNA in the cerebrospinal fluid of children with herpes simplex encephalitis." J Med Virol. 41. 170-173 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimura H et al.: "T-cell receptor V a region in the cerebrospinal fluid of patients with mumps meningitis." J Med Virol. 41. 306-311 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 森島 恒雄: "単純ヘルペスウイルス" 脳と発達. 25. 124-127 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morishima T: "New diagnostic techniques for the management of herpesviruses." HERPES. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 森島 恒雄: "新しい集中治療の指針 全身感染症(P353〜357)" 南山堂(吉矢生人、島田康弘編), 415 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 森島 恒雄: "微生物学コメンタリー ウイルス学各論(P192〜195)" 中外医学社(平松啓一,南嶋洋一編), 236 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osuga T,Morishima T,Hanada N,Nishikawa K,Isobe K,Watanabe K.: "Transfer of specific IgG and IgG subclasses to herpes simplex virus across the blood-brain barrier and placenta in preterm and term newborns." Acta Paediatr. 81. 792-796 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimura H,Aso K,Kuzushima K,Hanada N,Shibata M,Morishima T.: "Relapse of herpes simplex encephalitis in children." Pediatr. 89. 891-894 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ando Y,Kimura H,Miwata H,Kudo T,Shibata M,Morishima T.: "Quantitative analysis of herpes simplex virus DNA in the cerebrospinal fluid of children with herpes simplex encephalitis." J Med Virol. 41. 170-173 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimura H,Ando Y,Shibata M,Abe T,Morishima T.: "T-cell receptor V a region in the cerebrospinal fluid of patients with mumps meningitis" J Med Virol. 41. 306-311 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morishima T.: "Herpes simplex virus" Brain and Development. 25. 124-127 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morishima T.: "New diagnostic techniques for the management of herpesviruses." HERPES. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morishima T.: New treatment in ICU. Nanzando(edited by Yoshiya I and Shimada Y), 353-357 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morishima T.: Commentary of Microbiology. Chugaiigakusha(edited by Hiramatsu K and Minamishima, 192-195 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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