1993 Fiscal Year Final Research Report Summary
Immunological study about the role of activated eosinophjils in tumor regression
| Project/Area Number |
04670738
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
IWASAKI Kazunori Kyusyu University, Research Associate, 医学部, 助手 (10150533)
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| Co-Investigator(Kenkyū-buntansha) |
TORISU Motomichi Kyusyu University, Associate Professor, 医学部, 助教授 (90038810)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Eosinophil / LAK / IL-5 / Chemotaxis / CDllb / ADCC |
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| Research Abstract |
We previously found that eosinophilia could often be detected in peripheral blood from patients with lung or esophageal cancer. Eosinophils from these patients showed the same percentage of hypodense population with those from healthy volunteer. Is is well known that the number of eosinophils and percentage of hypo-dense population significantly increase in peripheral blood from cancer patints aferr LAK and IL-2 therapy.
To determine the mechanism by which eosinophils are activated after the therapy, we examined the functional changes of human eosinophils induced by the supernatant of LAK cell cultures. The chemotactic activity of eosinophils in the supernatant of LAK cell cultures was increased significantly, and this activity was chemotactic rather than chemokinetic by checcurboard analysis. Both chemiluminescence and superoxide production of stimulated eosinophils increased compared with resting. Among leukocyte integrins, CDllb expression on stimulated eosinophils increased. Moreover, eosinophil antibody-dependent cellular cytotoxicity against Raji cells was enhanced after stimulation with the supernatant of LAK cells. We found that IL-5 activity which is one of potent eosinophil-activating factors was significantly increased in LAK supernatant. An in vitro study demonstrated that IL-5 enhanced tumoricidal activity of LAK cells as wel as cosinophils. These results suggest that IL-5 which is produced by LAK cells play a role in activation of eosinophils in LAK therapy and possibly anti-tumor effect.
Taken together our results suggest that activated eosinophils induced by LAK therapy may play a role in anti-tumor effect in this therapy. IL-5 in combination with LAK therapy could be used for anti-cancer adaptive immunotherapy.
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