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1995 Fiscal Year Final Research Report Summary

Quantatative analysis of laminin and laminin binding protein mRNA in patients with a colorectal cancer

Research Project

Project/Area Number 04670792
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionOita Medical University

Principal Investigator

MIYAHARA Masaki  Oita Medical Univ. Assistant Prof., 医学部, 講師 (10183641)

Co-Investigator(Kenkyū-buntansha) SHIMODA Katsuhiro  Oita Medical Univ. Lecturer, 医学部, 助手 (90211292)
Project Period (FY) 1992 – 1994
KeywordsColorectal cancer / Hepatic metastasis / Laminin / Laminin binding protein / mRNA / Northern blotting / MMP / TIMP
Research Abstract

The expression of laminin A chain, B1 chain, B2 chain and 32kd laminin binding protein (LBP 32) mRNAs in the cancer tissue and normal colonic tissue were analyzed by Northern blot technique in patients with colorectal cancer.
1. laminin mRNA expression (17 cases) : laminin mRNA expression was detected in the normal colonic tissues with the DNA probe of laminin B1 chain, but not detected in the cancer tissues with any laminin DNA probes.
2. LBP 32 mRNA expression (64 cases) : LBP 32 mRNA expression was detected in cancer and normal tissues examined. The mean value of LBP 32 mRNA/beta-actin mRNA of cancer tissues was significantly higher than that of normal tissues (p<0.005). High expression of LBP 32 mRNA was correlated with depth of cancer invasion (p<0.05), venous invasoin and hepatic metastasis (not significant statistically). No correlationship was recognized between high expression of LBP 32 mRNA and distant recurrence after 44 curative operations.
In connection with the theme, we analyzed the mRNA expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPS) in 66 cancer and normal tissues of colorectal patients in this project. There were significant correlation between mRNA expression level of MMP-9 and that of TIMP-1 and TIMP-2. High mRNA expression of TIMP-1 and TIMP-2 was significantly correlated with Dukes classification.

  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 村重光哉: "大腸癌におけるTIMP-1の発現とその臨床的意義" 消化器癌の発生と進展. 5. 217-219 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsuya Murashige: "Enhanced expression of tissue inhibitors of metalloproteinases in human colorectal tumors" Jpn J Clin Oncol. 6(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsuya Murashige.: "Enhanced expression of tissue inhibitors of metalloproteinases in human colorectal tumors" Jpn J Clin Oncol. 6(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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