• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1993 Fiscal Year Final Research Report Summary

In vivo observation of coronary microcirculation using a needle-probe video-microscope with a CCD camera during cardiac surgery

Research Project

Project/Area Number 04670844
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Thoracic surgery
Research InstitutionKawasaki Medical School

Principal Investigator

FUJIWARA Takashi  Kawasaki Medical School, Thoracic Surgery, Professor, 医学部, 教授 (90090224)

Co-Investigator(Kenkyū-buntansha) GOTO Masami  Kawasaki College of Allied Health Professions, Medical Electronics, Associate Pr, 医用電子技術科, 助教授 (50148699)
OGASAWARA Yasuo  Kawasaki Medical School, Medical Engineering, Assistant Professor, 医学部, 講師 (10152365)
TSUJIOKA Katsuhiko  Kawasaki Medical School, Medical Engineering, Associate Professor, 医学部, 助教授 (30163801)
KAJIYA Fumihiko  Kawasaki Medical School, Medical Engineering, Professor, 医学部, 教授 (70029114)
Project Period (FY) 1992 – 1993
KeywordsCCD microscope / Coronary circulation / Thoracic surgery / Microcirculation / Reactive hypermia / Subendocardial microvessel / Nitroglycerin
Research Abstract

To evaluate the microcirculation of the human myocardium morphologically and functionally, we developed a portable needle-probe video-microscope with a charge coupled device (CCD) camera was applicable to the coronary microvessels during a cardiac surgery. First, using open-chest anesthetized pigs and dogs, we aimed to find technological hurdles for introducing the system during a surgery. Then, we developed several pieces of equipment to overcome the methodological problems. In brief, a sheathed needle-probe with a doughnut-shaped balloon was developed to avoid direct compression of the microvessels by the tip of the needle. To maintain a physiological condition of myocardial microvessels and the surrounding tissue during observation, a system consisting of a heat exchanger and a microtube was developed for flushing away the intervening blood between the tip of the needle and the myocardium. Using these devices, images of the myocardial microvessels of a beating heart could be obtained for a time which was long enough for imaging study. Furthermore, a holding device of the portable needle-probe video-microscope with a CCD camera was developed, which allowed an operator easy handling. This holding device contributed to shorten observation time. For the myocardial microvessels to be visualized without any special intravascular contrast medium, we employed a green (complimentary color of red) filter to accentuate the contrast between the images of the blood filled vessel and the surrounding tissue. Then, not only the coronary arterioles, but also coronary venules could be visualized. We confirmed that the portable needle-probe video-microscope with a CCD camera was able to visualize human coronary microvessels using all these supporting systems.
We also evaluated the effects of nitroglycerin microvessels. The study showed that nitroglycerin dilates both subepicardial subendocardial arterioles. It was also found that nitroglycerin dilated coronary venules.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 藤原巍、他: "冠動脈再建術における静脈グラフトおよび動脈グラフトの選択" 脈管学. 32. 1081 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小笠原康夫、他: "ニードル型CCD顕微鏡による心筋壁内膜側微小血管の観察" JAMIT,92研究報告. 31-34 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toyotaka Yada,et al.: "In vivo observation of subendocardial microvessels of the beating porcine heart using a needle-probe videomicroscope with a CCD camera" Circulation Research. 72. 939-946 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fumihiko Kajiya,et al.: "Velocity profiles and phasic flow patterns in the non-stenotic human left anterior descending coronary artery during cardiac surgery" Cardiovascular Research. 27. 845-850 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsuhiko Tsujioka,et al.: "In-vivo observation of subendocardial arteride and venule by a needle-probe videomicroscope with a CCD camera in a beating heart" Abstracts of 2nd Far Eastern Conference on Medical and Biological Engineering. 284 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osamu Hiramatsu,et al.: "Effect of cardiac cortraction on the perforated intramural arteriole and venule in the dog" Circulation. 88. 1757 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takashi Fujiwara, et al.: "Coronary ostial endarterectomy in Takayasu's aortitis-Confirmation of patency nine years postsurgically" Japanese Circulation Journal. 56. 556-559 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasuo Ogawsawara et al.: "Blood velocity profile in poststenotic region and velocity waveform of coronary arterial inflow on to intramyocardium during coronary artery stenosis in dogs" Proceedings of the 14th Annual Interntional Conference of the IEEE Engineering in Medicine and Biology Society. 91-92 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masami Goto, et al.: "Effect of intracorocnary nitroglycerin administration on phasic pattern and transmural distribution of flow during corocnary artery stenosis" Circulation. 85. 2296-2304 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toyotaka Yada, et al.: "In vivo observation of subendocardial microvessels of the beating porcineheart using a needle-probe videomicroscope with a CCD camera" Circulation Research. 72. 939-946 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fumihiko Kajiya, et al.: "Velocity profiles and phasic flow patterns in the non-stenotic human left anterior descending coroanry artery during cardiac surgery" Cardiovascular Research. 27. 845-850 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsuhiko Tsujioka, et al.: "In-vivo observation of subendocardial arteriole and venule by a needle-probe videomicroscope with CCD camera in beating heart" Absrtacts of the 2nd Far Easten Conference on Medical and Biological Engineering. 284 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Osamu Hiramatsu, et al.: "Effect of cardiac contraction on the perforrated intramural arteriole and venule in the dog" Circulation. 88. 1757 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1995-03-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi