1994 Fiscal Year Final Research Report Summary

Increase in intracellular Ca^<2+> and neuronal injury

Research Project

Project/Area Number	04670874
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Cerebral neurosurgery
Research Institution	Juntendo University
Principal Investigator	ARAI Hajime Juntendo Univ. Medical Department Associate Professor, 医学部, 助教授 (70167229)
Co-Investigator(Kenkyū-buntansha)	KDOTA Yoshiaki Juntendo Univ. Medical Department Assistant, 医学部, 助手 (60204517) UDO Akira Juntendo Univ. Medical Department Assistant, 医学部, 助手 (10185051) YASUMOTO Yukimasa Juntendo Univ. Medical Department Assistant, 医学部, 助手 (20175649)
Project Period (FY)	1992 – 1994
Keywords	Hippocampus / Neuron / Glutamate / Calcium / Cell death / Hypothermia
Research Abstract	To elucidate the precise mechanism of neuronal protection by brain hypothermia, we conducted an investigation as to what effect low temperature had on glutamate-induced intracellular free calcium ([Ca^<2+>]i) accumulation and cell death in cultured hippocampal neurons. Fifteen minutes of exposure to either 100muM or 1mM glutamate uniformly induced a marked increase in [Ca^<2+>]i, with delayd recovery and massive neuronal death under the conditions of both 37ﾟC and 30ﾟC.The study indicated that brain hypothermia cannot save neurons once glutamate is released during ischemia, and that intraischemic hypothermia in vivo probably prevents the development of ischemic neuronal injuly by suppressing extracellular glutamate release.