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1993 Fiscal Year Final Research Report Summary

STUDIES ON PROPHYLAXIS AGAINST HYPEROXIC LUNG INJURY

Research Project

Project/Area Number 04670924
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 麻酔学
Research InstitutionKOBE UNIVERSITY

Principal Investigator

OBARA Hidefumi  KOBE UNIVERSITY SCHOOL OF MEDICINE.PROFESSOR, 医学部, 教授 (80030998)

Co-Investigator(Kenkyū-buntansha) MIKAWA Katsuya  KOBE UNIVERSITY SCHOOL OF MEDICINE.ASSISTANT PROFESSOR, 医学部・附属病院, 講師 (40229662)
MAEKAWA Nobuhiro  KOBE UNIVERSITY SCHOOL OF MEDICINE.ASSOCIATE PROFESSOR, 医学部, 助教授 (30127394)
Project Period (FY) 1992 – 1993
KeywordsOxygen Toxicity / Respiratory Failure / Reactive Oxygen Species / Pulmonary Surfactant / Superoxide Dismutase / Cytokines / Lidocaine / Neutrophil
Research Abstract

Hyperoxia causes acute lung injury, resulting, in humans, in adult respirtory distress syndrome. Neutrophils are thought to play a pivotal role in the pathogenesis of hyperoxic lung injury through the release of reactive oxygen species (ROS). Lidocaine and surfactant have been shown to inhibit production of ROS by neutrophils. The aim of this study was to determine whether pretreatment with lidocaine (L) or surfactant (SF) attenuated acute lung injury induced by hyperoxia in rabbits.
[Study 1]
The animals were divided into 3 groups : Group 1 : ventilation with air for 36 hr without L treatment, Group 2 : ventilation with 100% oxygen for 36 hr without L treatment, and Group 3 : ventilation with 100% oxygen for 36 hr with L treatment. In the L-treated group, a single i.v. dose of L (2 mg/kg) was administered immediately after the initiation of exposure to 100% oxygen, thereafter L was infused at a rate of 2 mg/kg/hr for 36 hr until the animals were sacrificed. The lungs of all rabbits were … More ventilated with 100% oxygen or air. Hemodynamics, PaO_2, and lung mechanics were recorded during the ventilation period. After exposure, the lung mechanics and cell fraction in bronchoalveolar lavage fluid (BALF) were measured and analyzed, as was activated complements (C3a and C5a), and cytokines (TNF and IL-1) in BALF.The lung wet-to dry-(W/D) weight ratio and albumin concentrations in BALF were analyzed as an index of pulmonary edema. We also compared light microscopic findings in the three groups. 100% oxygen for 36 hr caused no significant changes in hemodynamics, lung mechanics, or PaO_2/FiO_2 ratio. At the end of the 36-hr exposure period, hyperoxia significantly increased the lung W/D weight ratio, the influx of neutrophils into the lung, and C3a, C5a, TNF, IL-1, and albumin in BALF.L attenuated these increases. Exposure to 100% oxygen caused extensive morphologic lung damage, which was lessened by L.
[Study 2]
SF-treated rabbits were ventilated with 100% oxygen for 36 hr and SF (120mg/kg) was given via the trachea 12 hr after the start of mechanical ventilation. Saline-treated rabbits were treated idenically, except that they received saline without SF.There were no significant changes in hemodynamics, lung mechanics, or arterial oxygen tension during artificial ventilation.
Albumin and C3a in BALF and W/D weight ratio were higher in saline-treated group than in SF-treated group. Light microscopy revealed hyaline membrane formation in saline-treated rabbits, but fewer changes were observed in SF-treated group.
In conclusion, these results of Studies 1 and 2 suggest that intravenous L and intrabronchial SF have a prophylactic effect on initial hyperoxic lung injury (pulmonary vascular permeability, histopathological, and biochemical BALF changes) in rabbits. Less

  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Mikawa K.: "Perioperative changes in plasma C_3a and C_5a Concentrations in infants" Paediatric Anaesthesia. 2. 123-131 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mikawa K.: "Perioperative complement activation in neonates under halothane and fentanyl anaesthesia" Acta Anaesthesiol Scand. 36. 469-473 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishina K.: "Anesthetic considerations in a child with adrenoleulcodystrophy." Paediatric Anaesthesia. 3. 313-316 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikegaki J.: "Effects of surfactant on lung injury induced by hyperoxia and mechanical ventilation in rabbits." J.Anesth. 7. 68-74 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mikawa K.: "Efficacy of oral clonidine premedication in children" Anesthesiology. 79. 926-931 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mikawa K.: "Perioperative changes in superoxide production in neonates and infants" Can J Anaesth.40. 1162-1170 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Mikawa: "Perioperative changes in plasma C3a and C5a concentrations in infants." Paediaric Anaesthesia. 2. 123-131 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Shiga: "Anesthetic management of a child with Maroteau-Lamy syndrome." Journal of Anesthesia. 6. 344-348 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Mikawa: "Perioperative complement activation in neonates under halothane and fentanyl anaesthesia." Acta Anaesthesiologica Scandinavica.36. 469-473 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Mikawa: "A simple alternate technique for the application of the pulse oximeter probe to infants." Anesthesiology. 77. 400-401 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Ikegaki: "Effects of surfactant on lung injury induced by hyperoxia and mechaniacal ventilation in rabbits." Journal of Anesthesia. 7. 68-74 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Maekawa: "High frequency oscillation may be useful in perioperative respiratory management of neonates with congenital cystic adenomatoid malformation." Anesthesiology. 77. 1051-1052 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Nishina: "Anesthetic management of a patient with Cocayne's syndrome." Journal of Anesthesia. 7. 492-495 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Nishina: "Anesthetic considerations in a child with adrenoleukodystrophy." Paedistric Anaesthesia. 3. 313-316 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Nishina: "New technique to secure endotracheal tube using sports band for long-term respiratory management." Journal of Intensive Care Medicine. 8. 254 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Mikawa: "Improvement of gas exchange following endobronchial instillation of an exogenous surfactant in an infant with respiratory failure by postoperative pulmonary haemorrhage." Intensive Care Medicine. 20. 58-60 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Mikawa: "Efficacy of oral clonidine premedication in children" Anesthesiology. 79. 926-931 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Mikawa: "Selective intrabronchial instillation of a surfactant in a patient with pneumonia preliminary report." European Respiratory Journal. 6. 1563-1566 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Mikawa: "Perioperative changes in superoxide production in neonates and infants." Canadian Journal of Anaesthesia. 40. 1162-1170 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Obara: "Respiratory management for neonatal chronic respiratory failure (BPD)" Intensive and Critical Care Medicine. 4. 565-577 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Obara: "Point of long-term respiratory management in children." JOHNS. 8. 642-654 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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