1993 Fiscal Year Final Research Report Summary
Quantitation of oxygen radaical production of splanchnic organs in animals with various types of shock
| Project/Area Number |
04670942
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
麻酔学
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| Research Institution | Nippon Medical School |
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Principal Investigator |
OGAWA Ryo Nippon Medical School, Department of Anesthesiology, Professor, 医学部, 教授 (20008345)
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| Co-Investigator(Kenkyū-buntansha) |
尾藤 博保 日本医科大学, 医学部, 助手 (10246986)
YUUKI Teiichi Nippon Medical School, Department of Anesthesiology, Assistant, 医学部, 助手 (10174830)
YUUKI Teiichi Nippon Medical School, Department of Anesthesiology, Assistant
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| Project Period (FY) |
1992 – 1993
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| Keywords | hemorrhagic shock / splanchnic ischemia shock / lung injury by radicals / oxygen free radicals / spin-trapping techinique lipid peroxidartion / oxygen radical scavenger / スキャベンジャー |
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| Research Abstract |
Three models of organ injury induced by shock (hemorrhagic, splanchnic ischemia and lung injury) were made on adult rats. The methods were reported previously. New techniques were emplyed for trapping of radicals using PBN as a trapper. The details were reprted previously. Radaicals trapped into PBN were analyzed quantitatively using NSR spectrometry.
Spin aducts production in liver was increased to 270% after 2 hour of hemorrhagic shock. A small and tempoary elevation of spin aducts prodeuction in bowels was observed immediately after release of splanchinic artery occlusion, followed by reduction to control levels. Spin aducts production increased markedly after 30 minutes after reperfusion and show a peak at 90 minutes after in splanchnic ischemia shock model. On lung injury model radical production elevated linearly after injection of xanthine-xanthine oxidase solution into trachea. Lipid peroxidation increased pararelly to production of oxygen free radaicals in organ of animals subjected to 3 types of injury.
Oxygen radical scavengers such as allopurinol and superoxide dismutase were applied on animals with shock. Alopurinol did not show any effective insults, Superoxide dismutase showed a suppressive effect of oxygen radical production in splanchnic ischemia shock and lung injury models.
In rats which have no white blood corpuscle by pre-treatment of nitrogen mastard spinaducts production of bowel remained in a low state, suggesting a possible role of these blood cells in produciton of tissue injuries.
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