1994 Fiscal Year Final Research Report Summary
Studies on DNA topoisomerase-targeted anti-cancer chemotherapy in gynecologic malignancy.
| Project/Area Number |
04670995
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | GIFU UNIVERSITY |
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Principal Investigator |
NIWA Kenji Gifu Univ, School of Medicine, Research Associate, 医学部・附属病院, 助手 (80218247)
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| Co-Investigator(Kenkyū-buntansha) |
TAMAYA Teruhiko Gifu Univ, School of Medicine, Professor, 医学部, 教授 (70079870)
MISAO Ryo Gifu Univ, School of Medicine, Research Associate, 医学部・附属病院, 助手 (40262761)
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| Project Period (FY) |
1992 – 1994
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| Keywords | DNA topoisomerase I / mRNA / RT-PCR / Cancer cell lines / Camptothecin / Cytokines / anti-proliferative activity / Chemotherapy |
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| Research Abstract |
1. The anti-proliferative effects of INF-gamma and CPT-11, a new derivative of camptothecin, on two human endometrial cancer cell lines were examined in vitro. In both cell lines, INF-gamma enhanced the anti-proliferative activity of CPT-11.
2. Expression of DNA topoisomerase (Topo) -I mRNA in various cancer cell lines was detected using reverse transcription-polymerase chain reaction (RT-PCR) method. The cytoplasmic polyadenylated RNA isolated from cancer cell lines was reverse-transcribed and the complementary DNA was amplified by PCR primed with Topo-I specific primers. The level of Topo-I mRNA was correlated positively with the cytotoxicity of a Topo-I inhibitor.
3.The combination therapy of CPT-11 and INF-gamma will be examined in tumor-bearing mice or rats.
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