1993 Fiscal Year Final Research Report Summary
Interactions between cel cycle regulatory genes and anti-oncogenes contributed to the malignant potential of hydatidiform mole
| Project/Area Number |
04671002
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Osaka University |
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Principal Investigator |
OHASHI Kazutomo Osaka Univ. Med. School Dept. of Obstet. and Gyne. Instructor, 医学部, 助手 (30203897)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIURA Syouji Osaka Univ. Med. School Dept. of Obstet. and Gyne. Instructor, 医学部, 助手 (10243213)
KIMURA Tadashi Osaka Univ. Med. School Dept. of Obstet. and Gyne. Instructor, 医学部, 助手 (90240845)
AZUMA Chihiro Osaka Univ. Med. School Dept. of Obstet. and Gyne. Instructor, 医学部, 助手 (20151061)
MATSUZAKI Noboru Osaka Univ. Med. School Dept. of Obstet. and Gyne. Instructor, 医学部, 助手 (30199781)
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| Project Period (FY) |
1992 – 1993
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| Keywords | hydatidiform mole / choriocarcinoma / chrion / oncogenes / anti-oncogenes / gene mutation |
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| Research Abstract |
Interacition between cell cycle regulatory genes anti-oncogenes contributed to the malignant potential of hydatidiform mole.
1) Expression of oncogenes and anti-oncogenes in hydatidiform mole, choriocarcinoma, and choriocarcinoma cell lines.
Northern blot analyzes have revealed elevated expression of several oncogenes and decreased expression of a few anti-oncogenes in hydatidiform mole, choriocarcinoma, and choriocarcinoma cell lines. DNA deletions of a few anti-oncogenes were also detected in hydatidiform mole by southern blot analyzes. In order to detect point mutaions of oncogenes and anti-oncogenes, SSCP method was performed. cDNA of those tumors was synthesezed and used for SSCP.SSCP has revealed several point mutations in those tumors. Those mutations were confirmed by sequencing method. Some of those mutations were detected in so-called hot spot. The others were not in hot spot.
2) Establishment of primaly culture system for early pregnant chorion.
Chorion was separeted in the trophoblast by collagenase prepatation. Primary culture system of separeted trophoblast on collangen coated petri-dish was established.
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