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1994 Fiscal Year Final Research Report Summary

Molecular Biological Study of HPV Oncogenes and Suppressor Genes in Cervical Carcino genesis

Research Project

Project/Area Number 04671004
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

NAGAI Nobutaka  Hiroshima Univ.School of Medicine, Assistant Professor, 医学部, 講師 (90198292)

Co-Investigator(Kenkyū-buntansha) MURAKAMI Takahiro  Hiroshima Univ.Medical Hospital Resident, 医学部・付属病院, 医員
OTA Sanae  Hiroshima Univ.Medical Hospital Research Associate, 医学部・付属病院, 助手 (10243552)
Project Period (FY) 1992 – 1994
KeywordsCervical Cancer / Human Papillomavirus / Oncogene / Tumor Suppressor Gene / Molecular Biology / Immuno Response
Research Abstract

Molecular biological studies have shown that human papilloma-virus (HPV), some oncogenes and tumor suppressor genes are associated with uterine cervical carcinogenesis.We examined HPV DNA typing and its gene expression, oncogenes (c-myc, EGF-R) and p53 in cervical dysplasia and cancer with molecular biologic, immunohistochemical technique and binding assay.
The HPV study revealed that HPV type 16 and 18 DNA as well as EGF-R was detected at a high frequency at a higher grade of dysplasia and in the early stage of cervical cancer.In the oncogene study, c-myc gene overexpression was recongnized in the advanced stage of cervical cancer.The other oncogene and p53 were found in a low frequency and were non-specific genes in cervical cancer.In analysis of local immune, the number of S-100 positive cells and CD45RO cells increased as the grade of dysplasia was higher.
These findings indicated that HPV type 16/18 promote DNA synthesis throughout EGF-R and playd a role of initiation in cervical carcinogenesis and that some oncogenes and tumor suppressor genes were associated with cervical cancer.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 永井宣隆: "子宮頚癌の遺伝子診断" 臨床病理. 42. 372-379 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 永井宣隆: "子宮頚癌におけるEpidermal Growth Factor Receptof(EGF-R)の発現" 産婦中四会誌. 43. 41-46 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagai N.: "Cytological analysis foy human papillomarirus DNAs in cervical intraepithelial neoplasia by in situ hybridization" Hiroshima.J.Med.Sci.43. 105-110 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 永井宣隆: "子宮頚部腺癌におけるHPV16型、18型感染と癌抑制遺伝子P53蛋白の検出" 癌の臨床. 40. 1219-1226 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 永井宣隆: "子宮頚癌におけるDNA ploidy patternとproliferating indexの検討-Flow cytometryによる解析-" 産婦中四会誌. 42. 149-152 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 村上隆浩: "子宮頚部HPV感染とproliferating cell nuclear antigenによる細胞増殖能との比較検討" 日本産科婦人科学会雑誌. 45. 967-972 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 太田さなえ: "子宮頚部、膣壁、外陰におけるHPV多発巣成果の分子生物学的検索" 日本産科婦人科学会雑誌. 44. 1931-1436 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷本博利: "子宮頚癌におけるC-myc遺伝子mRNA過剰発現に関する分子生物学的検討" 日本産科婦人科学会雑誌. 44. 566-570 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 永井宣隆: "RT-PCR法によるHPV初期遺伝子発現の検索" 医学のあゆみ. 161. 132- (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagai N. :"Detection of human papillomavirus type 16 and 18 DNA tumor suppressor gene p53 portein in uterine adenocarcinoma." Jpn.J.Cancer Clinic.40 :. 1219-1226 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagai N. :"Cytological analysis for human papillomavirus DNAs in cervical intaepithelial neoplasia by in situ hybridization." Hiroshima J.Med.Sci.43 :. 105-110 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagai N. :"Expression of epitherial grouth factor receptor in cervical cancer." The Chugoku & Shikoku District Journal Jpn.43 :. 41-46 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagai N. :"Gene diagnosis of uterine cervical cancer." Jpn.J.Clin.Pathol.42 :. 372-379 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagai N. :"DNA ploidy pattern and proliferating index in uterine carcinoma.-Analysis by flow cytometry-" The Chugoku & Shikoku District Journal Jpn.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murakami T. :"Comparative study of HPV infection and proliferating cell nuclear antigen of uterine cevix." Acta Obst.Gynaec.Jpn.45 :. 967-972 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ota S. :"Molecular biological study on multifocal infection of HPV in uterine cevix, vagina and vulva." Acta Obst.Gynaec.Jpn.44 :. 1431-1436 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanimoto H. :"Molecular biological study on the over expression of c-myc gene in uterine cervical carcinoma." Acta Obst.Gynaec.Jpn.44 :. 566-570 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagai N. :"Early gene expression of HPV byRT-PCR." Igaku no Ayumi Jpn.161 :. 132 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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