1994 Fiscal Year Final Research Report Summary
Studies on antiendometrial antibodies in endometriosis
| Project/Area Number |
04671014
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Yokohama City University |
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Principal Investigator |
GORAI Itsuo Yokohama City University, Sch.of Med., Dept.Obst.& Gyn., 医学部, 講師 (70162170)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAZAWA Tsuneo Yokohama City University, Sch.of Med., Dept.Obst.& Gyn., 医学部, 助手 (90254169)
HIRAHARA Fumiki Yokohama City University, Sch.of Med., Dept.Obst.& Gyn., 医学部, 講師 (30201734)
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| Project Period (FY) |
1992 – 1994
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| Keywords | endometriosis / antiendometrial antibody / ELISA / serum / peritoneal fluid / GnPH agonist |
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| Research Abstract |
Immunologic phenomena have been postulated in the etiology of endometriosis. The association of antiendometrial tissue-specific antibodies with endometriosis has been proposed, suggesting an abnormal polyclonal B-cell activation-a classic characteristic of autoimmune disease. Our aim was to investigate endometrial antigens involved in the autoimmunity of endometriosis. We first applied the ELISA method to detect antiendometrial antibodies in the serum and peritoneal fluid of endometriosis patients as well as normal females and found that antiendometrial antibody levels were sighificantly higher in the serum of endometriosis patients than those in normal controls without endometriosis (absorbance at 405nm : 0.414<plus-minus> 0.120 vs 0.249 <plus-minus>0.103). We could not find any differences in absorbance among groups with different r-AFS stages. The absorbance of 11 endometriosis patients decreased after treatment with GnRH agonist (0.791 to 0.656). Then we detected endometrial antigens against which autoantibodies directed with Western blotting. Thirteen (72.2%), 14 (77.8%), and 15 (83.3%) of 18 serum samples from endometriosis patients had antibodies reactive against endometrial antigen with MW of 26kd, 34kd, and 42kd, respectively, while 6 (33.3%), 8 (44.4%), and 8 (44.4%) of 18 samples from normal control women reacted against these antigens, respectively. The frequencies of antibodies to the endometrial antigens were significantly (p<0.05) higher in the endometriosis patients than in the normal control woman. Isolation and characterization of endometrial antigens might help further understanding of the pathogenesis of endometriosis as well diagnosis of endometriosis patients without laparoscopic intervent
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