1993 Fiscal Year Final Research Report Summary
The localization of beta 1,4-galactosyltransferase and its significance for malignancy
| Project/Area Number |
04671018
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
産婦人科学
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| Research Institution | Keio University |
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Principal Investigator |
AOKI Daisuke School of Medicine, Keio University, Assistant, 医学部, 助手 (30167788)
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| Co-Investigator(Kenkyū-buntansha) |
UDAGAWA Yasuhiro School of Medicine, Keio University, Assistant professor, 医学部, 講師 (80118918)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Galactosyltransferase / Tumor marker / Ovarian cancer / Immunofluorescence / Duble-determinant EIA / Immuno-dlectronmicroscopy / Site^directed mutagenesis |
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| Research Abstract |
In this study, 1) usefulness of galactosyltranseferase associated with tumor (GAT) as a tumor marker for ovarian tumors, 2) subcellular localization of galactosyltransferase(GalT), and 3) the mechanism for thetention of Galt in Golgi apparatus were investigated. Their outlines were described as follows ; 1) In order to measure GAT with double-denterminant enzyme immunoassay, we utilized monoclonal antibodies(Mabs) terned Mab8513 and Mab8628 generated against the GalT extracted from ascites of ovarian cancer patients. Assay of the serum-GAT levels showed favorite rate (52.9%) in ovarian cancers and extremely low false positive rate (5.7%) in benign tumors, indicating GAT to ve useful in terms of very low specificity for ovarian cancers. 2) Histochemical analysis using Mab8628 demonstrated a strong positive reaction in the suprasuclear area of cacer cells which coincided with trans-side of the Golgi apparatus as observed with immunoelectron microscopy. 3) Immunohistochemical expmination using Cos-1 cells transfected with wild and mutated cDNA for human galacrosyltransferase demonstrated that amino acid residues, Cys and His in a membrane-anchoring domain are necessaty for GalT to reside on the Golgi.
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