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1993 Fiscal Year Final Research Report Summary

ESTABLISHMENT AND CHARACTERIZATION OF HUMAN SPERM SPECIFIC MONOCLONAL ANTIBODY 1G1 WITH INHIBITORY EFFECT OF SPERM-ZONA PELLUCIDA INTERACTION

Research Project

Project/Area Number 04671026
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionHYOGO COLLEGE OF MEDICINE

Principal Investigator

KOYAMA Koji  HYOGO COLLEGE OF MEDICINE, DEPARTMENT OF OBSTETRICS AND GYNECOLOGY, PROFESSOR, 医学部, 教授 (00068496)

Co-Investigator(Kenkyū-buntansha) AKATANI Akiko  HYOGO COLLEGE OF MEDICINE, DEPARTMENT OF OBSTETRICS AND GYNECOLOGY, ASSISTANT PR, 医学部, 助手 (50212402)
YAMASAKI Noriyuki  HYOGO COLLEGE OF MEDICINE, DEPARTMENT OF OBSTETRICS AND GYNECOLOGY, ASSISTANT PR, 医学部, 助手 (50174644)
Project Period (FY) 1992 – 1993
Keywordsmonoclonal antibody / fertilization / ubiquitin / human sperm / 精子先体
Research Abstract

The MAb(1G1) possessing a strong fertilization-blocking activity was prepared by immunizing BALB/c with capacitated human sperm in order to study the molecular nature of sperm antigens relevant to fertilization. The MAb 1G1 strongly inhibited human sperm penetration into zona-free hamster eggs. It reacted to the apical portion of acrosome-reacted human sperm, but did not react to the live sperm before acrosome reaction, when the immunofluorescence staining were performed. In paraffin-embedded testis sections, the round spermatids, spermatocytes and spermatozoa were stained with the MAb 1G1. The spermatogonia, Sertoli cells, Leydig cells, and other somatic tissues were not stained. The sperm of monkeys, bulls, mice and boars were not stained. Therefore, the staining of sperm was species specific. The antigen corresponding to the MAb 1G1 showed a band at a 27kDa by immunoblotting. The reactivity of the antigenic components were not destroyed by periodic acid treatment. As a result, it was postulated that the antigenic molecule might be a peptide. After screening the human testis cDNA library with the MAb 1G1, we isolated a cDNA clone encoding the antigen. According to the nucleotide sequences of th cDNA clone, they showed that the cDNA contained human poly-ubiquitin DNA.These results suggests that ubiquitin might play an important role in human fertilization.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] Milena-Dubaua Mikalava et al: "Identification and chracterization of a 27KD acrosome protein of human sperm defined by monoclonal antibody with fertilization-blocking effect" Journal of Reproductive Immunology. (印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Milena Dubova-Mihailoval), Shinji Komori, Kinu Kameda, Yoshiyuki Tsuji, Koji Koyama, and Shinzo Isojima: "Identificcation and characterization of a 27 kD acrosome protein of human sperm difined by a monoclonal antibody with fertilization-blocking effect" Journal of Reproductive Immunology. in press. (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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