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1994 Fiscal Year Final Research Report Summary

Hoemeostais of inne ear on the point of non-specic defense

Research Project

Project/Area Number 04671047
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionOSAKA CITY UNIVERSITY

Principal Investigator

YAMANE Hideo  Osaka City University Medical School Assoc.professor, 医学部, 助教授 (60145787)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Kenichi  Osaka Prefectural College of Health Science Professor, 医療技術短期大学部, 教授 (40089959)
KONISHI Kazuo  Osaka City University Medical School Assis.professor, 医学部, 講師 (50145801)
Project Period (FY) 1992 – 1994
KeywordsInner ear / Cochlea / Stria vascularis / Non-specific defense / Free radical / NK cell / Acoustic trauma / K-562
Research Abstract

In this experiment ethiologically unnown iner ear disease has been studied to clarify the non-specific defense mechanism (first defense mechanism) of the inner ear against pathogen. K-562 myelogenous leukemia cells were injected into the guinea pig perilymphatic space through the round window membrane and the raction of the inner ear was observed on day 1 to day 9. NK cells which were positive for anti-asialo GM1 appared in the perilymphatic space on day 7 when most of K-562 cells were still intact. On day 9 there were many K-562 cells with adherent phlogocytes in the perilymph and were mostly degenerated. The emergence of NK cells was much later in the perilympahtic space than in the skin which was also injected with K-562 cells. And there is a possibility that this delay can lead to the irreversiable change of cochlea due to pathogens before the mobilization of NK cells. In addition, the humoral factors in the inner ear fluid was not so powerful in suppresing viral infection and the endolympohatic sac didnot seem to play an important role in the defense against this pathiogen to rescue the cochlea. In consequence, the inner ear seems to be an environment which is relatively difficult for immunocompetent cells to perform their functions.
The effect of acoustic trauma on cochlear strial circulation was investigated imuunohistologically in the guinea pig. Kanamycin was used as a tracer of blood flow. Besides, histochemical examination were made to ascertain the emergence of free radicals in the cochlea after acoustic trauma. The blood flow in the stria vascularis was greatly diminished. The cause of acoustic trauma is obscure. But superoxide anion radicals (O_2) may be related to strial blood cieculation and affect strial function.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 山根英雄、中井義明、小西一夫、坂本平守、井口広義、中川隆之、大和文博、高橋研一: "内耳の非特異的防御機構" Equilibiriun Res Sappl. 7. 281-286 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 井口広義、山根英雄、坂本平守、小西一夫、中川隆之、森中耕介、高山雅裕、中井義明: "内耳膜迷路の分裂と分化(第3報)" Otol Jpm. 2. 139 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 井口広義、山根英雄、坂本平守、小西一夫、中川隆之、高山雅裕、中井義明、中井義明: "蝸牛成熟過程についての検討" 日耳等. 95. 1629 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Yamane,Y.Nakai: "Imner ear damage due to lipoid nephrosis" Eur Arch Otorhinolarynagol. 250. 177-181 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 井口広義、山根英雄、小西一夫、坂本平守、中川隆之、森中耕介、高山雅裕、中井義明、高橋研一: "内耳の非特異的防御機構-第2報-" Otal Jpn. 3. 135 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山根英雄、中井義明、高山雅裕、小西一夫、井口広義、中川隆之: "音響暴露による蝸牛血流(とくに血管条血流)の変化" Otal Jpn. 3(3). 288-294 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 桜井敏忠雄、山根英雄、小西一夫、中井義明: "めまい統一評価基準私案" Equilibriun Res Suppl. 9. 126-132 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 井口広義、山根英雄、小西一夫、坂本平守、中川隆之、柴田太郎、高山雅裕、西村啓三、川勝さわ子、角田貴司子、中井義明、高橋研一: "内耳の非特異的防御機構-第3報-" Otal Jpn. 3. 615 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 井口広義、中井義明、山根英雄、: "内耳の局部防御機構に関する基礎的研究-特に非特異的防御機構について-" Eqailibriun Res Suppl. 10. 37-53 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Ymane,Y.Nakai,T.Nakagawa,K.Konishi,H.Iguchi,K.Morinaka,M.Takayama,H.CHO,K.Takahashi: "Metapolic activity and regeneration of avior hair cells" Proc.sendai Symposiun, 57-61 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Yamane,H.Iguchi,Y.Nakai,K.Konishi,T.Nakagawa,M.Takayama,K.Takahasi: "The first dcfense of the inner ear.Edo(G Mogi J.E.Veldman H Kaurudi)Immurobiology in Otorhinolaryngologic progress of decade" Karger, 165-174 (1994)

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      「研究成果報告書概要(和文)」より
  • [Publications] H.Yamane, Y.Nakai, T.Nakagawa, K.Konishi, H.Iguchi, K.Morinaka, M.Takayama, H.Cho, K.Takahashi: "Metabolic activity and regeneration" Ed (T.Takahashi) Proc.sendai Symposium. 92. 57-61 (1992)

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      「研究成果報告書概要(欧文)」より
  • [Publications] H.Yamane, Y.Nakai, K.Konishi, H.Sakamoto, H.Iguchi, T.Nakagawa, K.Morinaka, M.Takayama, F Yamato, K.Takahashi: "Non-specific defense of the inner ear" Equilibrium Res. 51 (2). 281-286 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Yamane, Y.Nakai, M.Takayama, K.Konishi, H.Iguchi, T.Nakagawa: "Strial blood circulation and acoustic trauma" Otol Jpn. 3 (3). 288-294 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Yamane, Y.Nakai: "Inner ear damage due to lipoid nephrosis." Eur Arch Otorhinolaryngol. 250. 177-181 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Yamane, H.Iguchi, Y.Nakai, T.Nakagawa, K.Konioshi, K.Nakagawa, M.Takayama, K.Takahashi: "The first defense of the inner ear." Immunobiology in otorhinolaryngology-Progress of Decade.PP165-174 Karger. (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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