1994 Fiscal Year Final Research Report Summary
Expression of metastasis-suppressor gene and its clinical application in the head and neck squamous cell carcinoma
| Project/Area Number |
04671059
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Research Institute, International Medical Center of Japan. |
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Principal Investigator |
ISHITOYA Jun-ichi Division of Biochemisty and Neutrition, Research fellow, 代謝疾患研究部, 研究員 (00191875)
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| Project Period (FY) |
1992 – 1994
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| Keywords | head and neck / squamous cell carcinoma / metastasis-suppressor gene / nm23 / サイトケラチン |
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| Research Abstract |
The nm23 gene was identified as a metastasis-suppressor gene in a murine melanoma cell line. Low nm23 mRNA level was found in human breast cancers from patients with evidence of lymph node metastasis. The nm23 gene product shows a very high homology to nucleoside diphosphate (NDP) kinase (Nm23/NDP kinase). We examined expression of Nm23/NDP kinase in the head and neck cancers immunohistochemically. Expression of Nm23/NDP kinase in the head and neck cancer was varied in both its intensity and intracellular distribution. Heterogeneity of the expression was observed even in the same sections. Cytokeratin (CK) -13 and CK-19 were reported to relate with cell differentiation in the head and neck cancers. We also examined expresison of CK-13 and CK-19 in the same tissues to compare with expression of Nm23/NDP kinase. Strong expression of Nm23/NDP kinase was observed in cells which expressed CK-13 but not CK-19. In contrast, reduced or no expression of Nm23/NDP kinase was associated to expressionn of CK-19. Therefore, level of expression of Nm23/NDP kinase was suggested to relate to differentiation of cancer cell. These results were compared with tumor size and nodal status. Expression of Nm23/NDP kinase was not associated with tumor size. However, there was an inverse relationship between Nm23/NDP kinase expression and nodal status. This study suggests that examination of Nm23/NDP kinase expression may be a factor predicting metastatic potential of the head and neck cancer.
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