Ocular hypotensive effect and intra-cellular signal transduction system of prostaglardins.

1993 Fiscal Year Final Research Report Summary

• Project/Area Number: 04671075
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: HIROSHIMA UNIVERSITY
• Principal Investigator: MISHIMA Hiromu Hiroshima Univ. Sch. of Med., Associate Prof., 医学部, 助教授 (20034100)
• Co-Investigator(Kenkyū-buntansha): KOSAKA Toshiya Hiroshima Univ. Medical Hospital, Research Associates, 医学部・附属病院, 助手 (50253084) ; KIUCHI Yoshiaki Hiroshima Univ. Sch. of Med., Research Associates, 医学部, 助手 (40214738) ; FURUMOTO Atsuhito Hiroshima Univ. Medical Hospital, Research Associates, 医学部・附属病院, 助手 (80238691) ; NIKAIDO Hirotoshi Hiroshima Univ. Sch. of Med., Research Associates, 医学部, 助手 (80237651)
• Project Period (FY): 1992 – 1993
• Keywords: Prostaglandins / Intra-cellular signaling system / Inositol triphospholipid (IP_3) / Cyclic AMP (_cAMP) / Ciliary epithelia / Blood aqueous barrier / Intraocular pressure

Research Abstract

Resently, prostaglandins (PG_s) have been identified as a new bio-active material. We attempted to clarify the relationships between PG_s and intracellular signaling systems, and the ocular hypotensive effect of PGF_<2alpha>. Then we investigated the effects of PG_s on blood aqueous barrier (BAB) and ciliary epithelia.
1. PGF_<2alpha> activates the inositol phospholipid turnover system, while PGD_2 and PGE_2 activate the _cAMP system.
2. Topical administration of phXA41, a prostaglandin F_<2alpha>-isopropyl ester analogue, caused marked intraocular pressure reduction in a dose-dependent manner in primary open-angle glaucoma patients. After long term apprication of phXA41 caused no aqueous flare rise, which means no effect on BAB.
3. There was no morphological changes detected in the iridial portion of ciliary processes in rabit's eye.

Research Products

• [Publications] Hotehema Y: "Clinical efficacy of PhXA34 and PhXA41,two novel prostaglandin F_<2α>-isopropyl ester analogues for glaucoma treatment." Jpn J Ophthalmol. 37. 256-269 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hotehema Y: "Ocular hypotensive effect of PhXA41 in patients with ocular hypertension or primary open-angle glaucoma." Jpn J Ophthalmol. 37. 270-274 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 小坂敏哉: "各種プロスタグランジン長期間点眼後の家兎毛様体の形態学的変化-毛様体突起虹彩部の変化-" 広島大学医学雑誌. 42(印刷中). (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 小坂敏哉: "各種プロスタグランディン長期間点眼後の前房蛋白濃度と家兎毛様体の形態学的変化." 日本眼科学会雑誌. 98(印刷中). (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hotehema Y: "Clinical efficacy of phXA34 and phXA41, two novel prostaglandin F_<2alpha>-isopropyl ester analogues for glaucoma treatment." Jpn J Ophthalmol. 37. 256-269 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hotehema Y: "Ocular hypotensive effect of phXA41 in patients with ocular hypertension or primary open-angle glaucoma." Jpn J Ophthalmol. 37. 270-274 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kosaka T: "The effects of topical application prostaglandins on the rabbit iridial portion of ciliary process : light and electron microscope sudies after the long-term application." Hiroshima M.J.42 (in press.). (1994)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kosaka T: "The effects of long-term topically applied prostaglandins on aqueous protein" Jpn J Ophthalmol Soc. 98, (in press.). (1994)
  • Description: 「研究成果報告書概要(欧文)」より