1993 Fiscal Year Final Research Report Summary
Analysis of Production of Epidermal Growth Factor in Submandibular Gland Controled by Hormons
| Project/Area Number |
04671129
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
OHARA Yuko Iwate Med Univ Sch Dent, Assistant, 歯学部, 助手 (10164667)
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| Co-Investigator(Kenkyū-buntansha) |
KYAKUMOTO Seiko Iwate Med Univ Sch Dent, Assistant, 歯学部, 助手 (90118274)
NEMOTO Takayuki Iwate Med Univ Sch Dent, Lecturer, 歯学部, 講師 (90164665)
SASAKI Minoru Iwate Med Univ Sch Dent, Lecturer, 歯学部, 講師 (40187133)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Gene Expression / Epidermal Growth Factor / Steroid / Submandibular Gland / Androgen |
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| Research Abstract |
A 6kb fragmant of the 5'-flanking region of the mouse EGF gene was investigated to characterize hormone response elements. A restriction map with 7 endonucleases was prepared and a series of chloramphenicol acetyltransferase reporter plasmids containing the 5'-flanking region-deletion mutants were constructed. An eukaryot expression vector of mouse androgen receptor was also constructed, and the expression of the receptor in COSI cells was confirmed by the enzyme-linked antibody-staining method with the anti-andogen receptor monoclonal antibody. We found a novel thyroid response element (EGF-TRE) at around position -250 : EGF-TRE matched the TRE of the Moloney leukemia virus gene by 67% in nucleotide sequence and competed specifically with the palindromic TRE for thyroid-receptor binding. The chloramphenicol acetyl-transferase assay also demonstrated a tyroid-dependent transcription activation within the region from position -400 to position -1. This region also responded to andorogen. These results suggest the existance of multicomponent regulatory complex in the EGF gene.
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