• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1994 Fiscal Year Final Research Report Summary

Effects of general anaesthetics and sedatives on central dopaminergic neuronal activity : a microdialysis study

Research Project

Project/Area Number 04671138
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionNihon University

Principal Investigator

KOSHIKAWA Noriaki  Nihon University, School of Dentistry, Associate Professor, 歯学部, 助教授 (80130491)

Co-Investigator(Kenkyū-buntansha) TAKADA Koji  Nihon University, School of Dentistry, Lecturer, 歯学部, 講師 (00216657)
KANAYAMA Toshiyoshi  Nihon University, School of Dentistry, Professor, 歯学部, 教授 (40224589)
Project Period (FY) 1992 – 1994
KeywordsStriatum / Nucleus accumbens / Dopamine release / Benzodiazepine receptor / Adenosine receptor / alpha_2-Adrenoceptor / Opioid receptor / Microdialysis
Research Abstract

Effects of benzodiazepine anaesthetics or sedatives on central dopaminergic neuronal activity were studied by using in vivo brain microdialysis to measure dopamine release from the striatum and the nucleus accumbens. Effects of the drugs often coadministered in clinic with the anaesthetics, i.e.clonidine and fentanyl, on central dopaminergic neuronal activity were also studied. Intravenous injections of midazolam and flunitrazepam decreased striatal dopamine release in a dose-dependent manner. Flumazenil also did not affect striatal dopamine release, but it prevented the effects of midazolam and flunitrazepam. Flunitrazepam also decreased striatal dopamine release when infused into the striatum, but it failed to affect the dopamine release when infused into the ipsilateral substantia nigra. Central administrations of midazolam were effective only when the drug was infused into both sites simultaneously. Intravenous injections of midazolam also dose-dependently decreased dopamine release from the nucleus accumbens. In contrast, the benzodiazepine receptor inverse agonist, beta-CCE,dosedependently increased the dopamini release. The effects of both drugs were prevented again by flumazenil. Moreover, adenosine receptors were not suggested to be involved in the benzodiazepine receptor-mediated dopamine decrease from the nucleus accumbens. Clonidine, an alpha_2-adrenoceptor agonist, decreased dopamine release from the nucleus accumbens, and enhanced the effects of midazolam. The results are suggestive of clinical use of clonidine to reduce the anaesthtic requirement of midazolam when used as a premedicant. Fentanyl, an opioid analgesic, increased dopamine release from the nucleus accumbens in a does-dependent manner. The effects were suggested to be mediated by the nucleus accumbens mechanisms. Furthermore, the effects of fentanyl was inhibited by midazolam infused into the nucleus accumbens but not infused into the ventral tegmental area.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Koji,Takada: "Effects of midazolam and flunitrazepan on the release of dopamine from rat striatum measured by in vivo microdialysis" British Journal of Anaesthesia. 73. 181-185 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tameatsu Murai: "Opposite effects of midazolam and β-carboline-3-carboxylate ethyl ester on the release of dopamine from rat nucleus accumbens measured by in vivo microdialysis" European Journal of Pharmacology. 261. 65-71 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takada, K., Murai, T., Kanayama, T.and Koshikawa, N: "Effects of midazolam and flunitrazepam on the release of dopamine from rat striatum measured by in vivo microdialysis" British Journal of Anaesthesia. 70. 181-185 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murai, T., Koshikawa, N., Kanayama, T.Takada, K.and Kobayashi, M: "Opposite effects of midazolam and -carboline-3-carboxylate ethyl ester on the release of dopamine from rat nucleus accumbens measured by in vivo microdialysis" European Journal of Pharmacology. 261. 65-71 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1996-04-15  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi