1993 Fiscal Year Final Research Report Summary
Experimental study on ostenoinduction of bio absorbable polymer-bone morphogenetic protein composite.
| Project/Area Number |
04671161
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
SHINTANI Hideki Hiroshima Univ. School of Dentistry, Professor, 歯学部, 教授 (80034239)
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| Co-Investigator(Kenkyū-buntansha) |
URABE Hidenori Hiroshima Univ. School of Dentistry, Instructor, 歯学部・附属病院, 助手 (10231185)
SATOU Naoki Hiroshima Univ. School of Dentistry, Associates Professor, 歯学部, 助教授 (20136097)
SHINTANI Hideki Hiroshima Univ. School of Dentistry, Professor (80034239)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Bioabsorbable poymer / Bone morphogenetic protein / Polylactic acid / Polyglycolic acid / Obteoinduction |
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| Research Abstract |
This study undertaken to evaluate the usefulness of bioabsorbable polymer (BAP) as a crrier of bone morphogenetic protein (BMP). Three kinds of granular BAPs (two polylactic acids with different molecular weight and lactic-glycolic acid copolymer) were implanted into rat tibiae and light microscopic sections were prepared following surgery. BAPs were gradually absorbed and replaced by bone marrow tissue by 24 weeks. Phagocytic reactions to the BAPs were noted, however, severe inflammation was not seen. Nextly, BAPs were mixed with demineralized bone particles prepared from rat femurs and laid subcutaneously. Histological examination revealed cartilage formation at 2 weeks and new bone formation at 4 weeks. Extensive bone and marrow formation were observed at 24 weeks. These results demonstrate that good tissue compatibility of BAP at the skeletal site and it dose not have negative influence on the osteinductive activity.
Crude BAP was extracted from the rat demineralized bone powder by 4M Guanidine HCl. BMP was mixed with BAP, then implanted in rat subcutaneously.New bone trabeculae with the BAP granulues occured at 2 weeks. Histomorphometrical analysis revealed that the amount of new bone of the BAP-BMP composite was more than of the BMP alone.
These results indicate that BAP is effective as a carrier of BMP.
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