1994 Fiscal Year Final Research Report Summary
特異的抗腫瘍効果を期待した放射線療法との併用による新しい養子免疫療法の確立
| Project/Area Number |
04671246
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
外科・放射線系歯学
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
KOSE Akira Kanagawa Dental College, School of Dentistry, assistant professor., 歯学部, 講師 (70215268)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUGAWA Shuji Kanagawa Dental College, School of Dentistry, Instructor., 歯学部, 助手 (70229459)
MATSUMOTO Goich Kanagawa Dental College, School of Dentistry, Instructor., 歯学部, 助手 (60199867)
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| Project Period (FY) |
1992 – 1994
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| Keywords | Radiation / IFN-gamma / LAK cell / MHC class I antigen / Oral squeamous cartinoma / Immunotherapy |
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| Research Abstract |
The purpose of this study is buildup adoptive immunotherapy effect with increase of MHC class I antigen on target cells by radiation.
1.Increase of MHC class I Antigen on Four Kinds of Cell Lines of Human Squamous Cell Cartinoma
MHC class I antigen on cell lines of human squamous cell cartinoma increase by radiation and/or interferon-gamma (IFN-gamma).
2.Changes of Lymphokine Activated Killer (LAK) Sensitivity with Increase of MHC class I Antigen on Cell Lines of Human Squamous Cell Cartinoma
The sensitivity of cytotoxicity against human allogenic LAKcells is decreased with increase of MHC class I antigen.
3.Increase of MHC class I Antigen on Murine Cell Line of Squamous Cell Cartinoma NRS1
MHC class I antigen on NRS1 increased by radiation and/or IFN-gamma.
4.Changes of LAK Sensitivity with Increase of MHC class I Antigen on NRS1
The sensitivity of cytotoxicity against murine syngenic (C3H/He) and allogenic (C57BL/6, Balb/c) LAK cells is decreased with increase of MHC class I antigen on NRS1.
5.Elimination of MHC class I Antigen on NRS1
MHC class I antigen on NRS1 disappeared by treatment of pH3.0 reagent.
6.Changes of LAK Sensitivity with elimination of MHC class I antigen
The sensitivity of cytotoxicity against murine allogenic (C57BL/6, Balb/c) LAK cells is increased with elimination of MHC class I antigen on NRS1.
7.Investigation for Subpopulation in LAK Cells which take part in the Changes of LAK Sensitivity
It suggest that asialo-GM1 antigen positive cells participate in the change of LAK sensitivity dependent on MHC class I antigen of NRS1 in this study.
we are investigatingthe role of of CD8 antigen positive cells in the change of LAK sensitivity.
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