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1993 Fiscal Year Final Research Report Summary

The mechanism of cell cycle G2 phase in mammalian cells

Research Project

Project/Area Number 04671393
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 医学一般
Research InstitutionThe University of Tokyo (1993)
Osaka University (1992)

Principal Investigator

NAGATA Akihisa  The University of Tokyo, Faculty of Medicine, Instructor, 医学部(医), 講師 (50155933)

Co-Investigator(Kenkyū-buntansha) OKAYAMA Hiroto  The University of Tokyo, Faculty of Medicine, Professor, 医学部(医), 教授 (40111950)
Project Period (FY) 1992 – 1993
KeywordsCell cycle G2 phase / Cdc25 tyrosine phosphatase / Cdc2 kinase
Research Abstract

The cdc25^+ tyrosine phosphatase is a key mitotic inducer of the fission yeast Schizosaccharomyces pombe, controlling the timing of the initiation of mitosis. Mammals contain at least three cdc25^+ homologues called cdc25A, cdc25B and cdc25C.We investigate the biological function of cdc25A.Although very potent in resucuing the S.pombe cdc25 mutant, cdc25A is less structurally related to the S.pombe enzyme. Northern and Western blotting detection reveals that unlike cdc25B, cdc25C and cdc2, cdc25A is predominantly expressed in late G1. Moreover, immunodepletion of cdc25A in rat cells by microinjection of a specific antibody effectively blocks their cell cycle progression from G1 into the S phase as determined by laser scanning single cell cytometry. These results indicate that cdc25A is not a mitotic regulator but a novel phosphatase that plays an crucial role in the start of the cell cycle. In view of its strong ability to activate cdc2 kinase and its specific expression in late G1, cdc2-related kinases functioning early in the cell cycle may be targets for this phosphatase.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Honda Reiko: "Dephosphorylation of human p34^<cdc2> kinase on both Thr-14 and Tyr-15 by human cdc25B phosphatase" FEBS Letter. 318. 331-334 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamura Kazuyoshi: "CyclinG:a new mammalian cyclin with homology to fission yeast Cigl" Oncogene. 8. 2113-2118 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jinno Shigeki: "Cdc25A is a novel phosphatase functioning early in the cell cycle" EMBO J in press. 6. (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 永田昭久: "細胞工学4" 秀潤社, 318 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 永田昭久: "蛋白質核酸酵素11" 共立出版, 96 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] HONDA REIKO: "Dephosphorylation of human p34^<cdc2> kinase on both Thr-14 and Tyr-15 by human cdc25B ohosphatase." FEBS Letter. 318. 331-334 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TAMURA KAZUYOSHI: "Cyclin G : a new mammalian cyclin with homology to fission yeast Cigl." Oncogene. 8. 2113-2118 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] JINNO SHIGEKI: "Cdc25A is a novel phosphatase functioning early in the cell cycle." (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] NAGATA AKIHISA: SAIBOUKOUGAKU 4. SHUUJYUNNSHA, 318 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] NAGATA AKIHISA: TANNPAKUSITU KAKUSANN KOUSO 11. KYOURITUSYUTPANN, 96 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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