1994 Fiscal Year Final Research Report Summary
Molecular analysis and therapy of diabetic angiopathy
| Project/Area Number |
04671457
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内分泌・代謝学
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| Research Institution | University of Tsukuba |
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Principal Investigator |
OKUDA Yukichi Inst. of Clin. Med., University of Tsukuba Associate professor, 臨床医学系・内科, 助教授 (60211132)
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| Project Period (FY) |
1992 – 1994
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| Keywords | Diabetic Angiopathy / Vascular endothelial cell / Aortic Smooth muscle cell / High glucose / Myo-inositol / Eicosapentaenoic acid / PDGF / Nitric oxide |
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| Research Abstract |
Hyperglycemia is believed to be the major cause of diabetic vascular complications. We evaluated the effect of high glucose on polyol mechanism, PDGF production in human umbilical vein endothelial cells (HUE) and aortic smooth muscle cell (ASMC). (1) Myo-inositol incorporation into endothelial cells may be dependent on an active transport system via Na^+-K^+-ATPase activity. (2) Eicosapentaenoic acid (EPA) and Aldose-reductase inhibitor (ONO 2235) decreased glucose mediated inhibition of myo-inositol uptake. (3) Under excess of ambient glucose (13.8,27.5 mM) and a hyperosmolar condition (122.0 mOsm/L with mannitol), PDGF in culture medium were both significantly incresed. Parallel to protein secretion levels, PDGF-B chain mRNA levels showed a significant increase. (4) EPA enhanced NO production in HUE.Also EPA inhibited migration of ASMC induced by PDGF.(5) After administration of EPA,Lipo PGE_1, the blood flow of the lower extremity of diabetic patients significantly increased.
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[Publications] Okuda Y., Mizutani M., Ogawa M., Sone H., Asano M., Tsurushima Y., Morishima Y., Asakura Y., Suzuki S., Isaka M., Sakakibara Y., Yamashita K., Hori M.: "Hemodynamic effects of lipo-PGE_1 on peripheral artery in patients with diabetic neuropathy : evaluated by two-dimensional color doppler echography."
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「研究成果報告書概要(欧文)」より
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[Publications] Sone H., Okuda Y., Mizutani M., Asakura Y., Asano M., Tsurushima Y., Ogawa M., Yamaoka T., Suzuki S., Isaka M., Bannai C., Kawakami Y., Odawara M., Matsushima T., Kawai K., Yamashita K.: "Effect of ibudilaston on lower limb circulation in NIDDM patients - Evalueted by doppler echography and laser blood flowmetry -" J.Japan Diab. Soc.37 (4). 307-310 (1994)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Asano M., Okuda Y., Mizutani M., Sone H., Tsurushima Y., Ogata M., Asakura Y., Yamaoka T., Suzuki S., Isaka M., Bannai C., Kawakami Y., Odawara M., Matsushima T., Kawai K., Yamashita K.: "Effects of beraprast sodium on lower limbcirculation in NIDDM patients - Evaluated by doppler echography and laser blood flowmetry -" J.Japan Diab. Soc.37 (5). 379-382 (1994)
Description
「研究成果報告書概要(欧文)」より
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