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1993 Fiscal Year Final Research Report Summary

GENERATION OF NK CELLS FROM CD34+ HEMATOPOIETIC STEM CELLS

Research Project

Project/Area Number 04671508
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionTHE INSTITUTE OF PFYSICAL AND CHEMICAL RESEARCH (1993)
University of Tsukuba (1992)

Principal Investigator

NAKAUCHI Hiromitsu  LABORATORY OF CELL GROWTH AND DIFFERENTIATION, THE INSTITUTE OF PHYSICAL AND CHEMICAL RESEARCH, TEAMLEADER, 造血制御研究チーム, チームリーダー (40175485)

Co-Investigator(Kenkyū-buntansha) NAKAUCHI Hiromitsu  LABORATORY OF CELL GROWTH AND DIFFERENTIATION, THE INSTITUTE OF PHYSICAL AND CHE
Project Period (FY) 1992 – 1993
KeywordsHematopoietic stem cells / NK cells / Stem Cell Factor / IL-2 / IL-3 / stroma cells / CD34 / CD33
Research Abstract

NK cells are believed to play a role in the primary host defense against tumor cells and virus-infected cells. Although it is known that NK precursor cells reside in the bone marrow (BM), the developmental sequence and molecular mechanisms associated with their differentiation into NK cells remain controversial. In order to clarify the lineage relationship between NK cells and other hematolymphoid cells, a cell culture system was established for human CD34^+ hematopoietic stem cells(HPSC). In medium containing both IL-2 and stem cell factor (SCF), NK cells differentiated from FACS-sorted CD34^+ HPSC, and when CD34^+ HPSC were fractionated according to CD33 expression, NK cells were obtained only from CD34^+CD33^+ cells. By contrast, CD34^+CD33^- cells grown in medium supplemented with IL-3 and SCF for 7 days before exposure to IL-2 also differentiated into NK cells after an additional 21 days of culture. These data provide direct evidence that NK cells arise from HPSC and reveal the identity of the cytokines required for their differentiation.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Yuji Gunji,et.al.: "Expression and function of adhesion molecules on human hemopoietic stem cells:CD34+LFA-1-cells are more primitive than CD34+LFA-1+cells." Blood.80. 429-436 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukio Nakamura,et.al.: "Two altemative forms of cDNA encoding CD34." Exp.Hematology.21. 236-242 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akira Shibuya,et.al.: "Enrichment of Interleukin-2-responsive Natural Killer progenitors in human bone marrow." Blood. 81. 1819-1826 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiromitsu Nakauchi et Gabriel Gachelin.: "Les cellules souches du sang." La Recherche.254. 537-541 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yuji Gunji,et.al.: "hematopoietic progenitor cells are more enriched in KITlow cells than in KIT high cells." Blood.82. 3283-3289 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akira Shibuya,et.al.: "IL-3,Stem Cell Factor and IL-2 cooperate in the generation of NK cells from the hematopoietic stem cell." (submitted).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yuji Gunji, Mitsuru Nakamura, Toshihiko, Hagiwara, Kayoko, Hayakawa, Hirohisa Matsushita, Hisao Osawa, Kazunari Nagayoshi, Hiromitsu Nakauchi, Masayoshi Yanagisawa, Yasusada Miura, and Toshio Suda: "Expression and function of adhension molecules on human hemopoietic stem cells : CD34^+LFA-1^- cells are more primitive than CD34^+LFA-1^+ cells" Blood.80. 429-436 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yukio Nakamura, Hajime Komano and Hiromitsu Nakauchi: "Two alternative forms of cDNA encoding CD34" Exp.Hematology. 21. 236-242 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akira Shibuya, Hiroshi Kojima, Kazuko Shibuya, Kazunari Nagayoshi, Toshio Nagasawa and Hiromitsu Nakauchi: "Enrichment of Interleukin-2-responsive Natural Killer progenitors in human bone marrow." Blood. 81. 1819-1826 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiromitsu Nakauchi et Gabriel Gachelin: "Les cellules souches du sang." La Recherche. 254. 537-541 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yuji Gunji, Mitsuru Nakamura, Hisao Osawa, Kazunari Nagayoshi, Hiromitsu Nakauchi, Yasusada Miura, Masayoshi Yanagisawa and Toshio Suda: "Human primitive hematopoietic progenitor cells are more enriched in KIT^<low> cells than in KIT^<high> cells." Blood. 82. 3283-3289 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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