1993 Fiscal Year Final Research Report Summary
Molecular and Pathological Analysis of Anticoagulant Heparan Sulfate Proteoglycan from Endothelial Cell
| Project/Area Number |
04671519
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General fisheries
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| Research Institution | Nagoya University |
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Principal Investigator |
TAKAMATSU Junki Nagoya University School of Medicine Associate professor, 医学部, 助教授 (80221365)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEO Takaaki Nagoya University School of Medicine Senior Resident, 医学部, 医員
TANIMOTO Mitsune Nagoya University School of Medicine Assistant professor, 医学部, 助手 (10240805)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Heparan Sulfate / Proteoglycan / Ryudocan / cDNA / Chromosomal Location |
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| Research Abstract |
We have isolated a series of overlapping cDNA clones encoding a 2,610 bp transcript, which potentially codes for a 198 amino acid protein with predicted molecular mass of 21,641 daltons, for the human ryudocan core protein. The deduced core proteins of the human and the rat ryudocan have high structural conservation, particularly in the NH_2 and COOH terminus regions of the putative mature core protein, including the combined transmembrane/cytoplasmic domains with conserved positions of all 4 tyrosine groups and 3 conserved glycosaminoglycan chain attachment regions, which might serve important roles for biological function of ryudocan. A major 2.7 kb transcript was detected in all tissues tested, with relatively high levels of expression observed in mRNA from lung, liver, skeletal muscle and kidney. A minor 1.9 kb transcript was also observed in some of tissues, which would be caused by alternative polyadenylation. Human ryudocan gene has been lacalized on the chromosome 20q12 by fluorescence in situ hybridization. Isolation of the human ryudocan core protein cDNA will allow us to study for the regulation of ryudocan expression and role of this molecule in human endothelial cell function.
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