1993 Fiscal Year Final Research Report Summary
Central role of IL-1 in the cytokine production in CSF-producing tumors
| Project/Area Number |
04671521
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
NAKAMURA Kishiko College of Medical Technology, Kyoto University, Instructor, 医療技術短期大学部, 助手 (20115900)
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| Project Period (FY) |
1992 – 1993
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| Keywords | CSF-producing tumor / IL-1 / IL-1 receptor antagonist / G-CSF / IL-6 / CAT assay / Transcription factor |
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| Research Abstract |
We have reported that tumours producing colony stimulating factor (CSF) secrete interleukin-1 (IL-1) and IL-6 in addition to CSFs. In the present study, we characterized the role of IL-1 in the cytokine production. IL-1 receptor antagonist inhibited production of G-CSF and IL-6 by around 90% in CSF-producing human lung carcinoma cell lines. Similar results were obtained with hydrocortisone which supresses IL-1 gene expression. In contrast, 15non-CSF-producing human lung carcinoma cell lines did not show detectable IL-1 production, although 7 of them were induced to produce G-CSF and IL-6 by exogenous IL-1alph. Cell lines which responded to IL-1alph including CSF-producing lines expressed receptors for IL-1alpha. From these results, CSF-producing tumours could be characterized by constitutive IL-1 production, IL-1 receptor expression and IL-1-dependent hyper-production of G-CSF and IL-6. Furthermore, the transcription factor(s) suggested as one of the cause of the abnormal IL-1alph production
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