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1994 Fiscal Year Final Research Report Summary

Involvement of nutrition and endocrinological system in muscle and bone atrophy : Relationship with aging

Research Project

Project/Area Number 04680094
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 家政学
Research InstitutionNagoya Women's University

Principal Investigator

KAWANO Setsuko  Nagoya women's Univ., Home Economics, Professor., 家政学部, 教授 (40024640)

Co-Investigator(Kenkyū-buntansha) SUEDA Kaori  Nagoya women's Univ., Home Economics, Associate Prof., 家政学部, 助教授 (60068278)
KAWANO Setsuko  Nagoya women's Univ., Home Economics, Professor. (40024640)
Project Period (FY) 1992 – 1994
KeywordsTail-Suspended Rat / Aging / Stress Hormones / Bone Atropy / Pyridinoline / Urinary Corticosterone / PTH / Vitamin D_3
Research Abstract

Disuse atrophy of musculo-skeletal system among aged people, is becoming a serious problem in many developed countries. It is thus mandatory to clarify the mechanism involved in the development of disuse atrophy and to invent countermeasures. Therefore, we investigated the mechanism involved in the disuse atrophy of hindlimb muscles and bones using the tail-suspension model in rats.
I) Studies using grwoing (5-8 week old) rats
Unloading of hindlimbs by tail-suspension for 1-2 weeks resulted in the remarkable atrophy of antigravity muscles and femur. Urinary excretion of pyridinoline was markedly increased as early as 1 day after the initiation of tail-suspension, suggesting that bone resorption is increased in the early stages of skeletal unloading. Indeed, activity of TR-ACP (tartarate resisting acid phosphatase) and serum free calcium concentration increased during the first three days of tail-suspension. Increased serum free calcium, probably due to bone resorption of hindlimb bones r … More esulted in the decreased secretion of PTH (parathyroid hormone). Since activation of vitamin D_3 (la-hydroxylation) is increased by PTH,the concentration of 1,25-(OH)_2-vitamin D_3 was also decreased during tail-suspension. The ALP (alkaline phosphatase activity) decreased after 5-day of tail suspension, suggesting that bone formation was suppressed by skeletal unloading at later stage.
In the tail-suspended rats, urinary excretion of corticosterone was increased from Day 1 to 3, indicating that suspension elicited stress response to the rats.
From these results, it is suggested that skeletal unloading causes early increase in bone resorption, resulting in the suppression of PTH secretion and decreased activation of vitamin D_3. Increased glucocorticoid secretion from the adrenocortical cells exerts strong catabolic action on muscle and bones. It is conclude that these combined response of endocrine system aggravates the disuse atrophy caused by skeletal unloading.
II) Study using aged (18 month old) rats
In the aged rats, tail-suspension for 1-2 weeks caused the atrophy of antigravity muscles as observed in the young rats. However, no atrophy of hindlimb bone was observed. Determination of ALP and TR-ACP activities in the femur revealed that the activity in the aged, non-suspended control was much less that of the young control rats. No alteration of the enzyme activities by tail-suspension was observed in the aged rats. These results suggest that bone remodeling in the aged rats is extremely low.
In the aged rats, urinary excretion of corticosterone was 15 times higher than that in the young rats even before the tail-suspension. Furthermore, no increase of corticosterone was observed after the initiation of tail-suspension, suggesting the alteration of hypothalamo-pituitary-adrenocortical regulation by aging. Less

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] 河野節子: "加齢がストレス反応に及ぼす影響:尾部懸垂ラットにおけるカテコラミン分泌反応の変化" 名古屋大学環境医学研究所年報. 46(in press). (1995)

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      「研究成果報告書概要(和文)」より
  • [Publications] Ito,T.: "Changes in Serum 1,25-Dihydroxyvitamin D_3 and mRNA_s for Osteocalcin and Alkaline Phosphatase in Femur Unloaded by Tail Suspension in Rats." Environ.Med.38. 103-106 (1994)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kawano,S.: "Adrenocortical response to tail-suspension in young and old rats." Environ.Med.38. 7-12 (1994)

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      「研究成果報告書概要(和文)」より
  • [Publications] 河野節子: "尾部懸垂ラットにおける尿中コルチコステロン排泄量およびハイドドロキシプロリン排泄量の変化:加齢の影響" 名古屋大学環境医学研究所年報. 45. 15-18 (1994)

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      「研究成果報告書概要(和文)」より
  • [Publications] 伊藤隆安: "ラット尾部懸垂における尿中骨代謝マーカーの変動-ピリジノリンとハイドロキシプロリンの比較" 名古屋大学環境医学研究所年報. 45. 12-14 (1994)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kawano S., Kanda K., Ohmori.S., Yamamoto C., Miyamoto N., Murata Y., Seo H.and Matsui N.: "Changes in urinary excretion of Ca and Pi and the bone metabolism in the tail-suspended rats-In relation with urinary excretion of corticosterone-" Ann.Res.Inst.Environ.Med., Nagoya Univ.43. 3-6 (1992)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Sakano S., Miyamoto N., Kawano S., Kanda K., Ohmori S., Murata Y., Seo H., Matsui N.and Miura T.: "Changes in mRNAs for Type I collagen and alkaline phosphatase in rat hindlimb bone by tail suspension" Ann.Res.Inst.Environ.Med., Nagoya Univ.43. 7-8 (1992)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kanda K., Ohmori, S., Yamamoto C., Miyamoto N., Kawano S., Murata Y., Matsui N.and Seo H.: "Urinary excretion of stress hormones of rats in tail-suspension." Environ.Med.37, No.1. 39-41 (1993)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Miyamoto N., Kanda K., Kawano S., Murata Y., Kambe F., Sueda K., Seo H.and Matsui N.: "Effect of adrenalectomy and orchiectomy of muscle atrophy of rat hindlimbs induced by tail suspension." Proceedings of the 3rd Inter-national symposium on Space Medicine in Nagoya 1992 (ed.Matsui, N.Seo.H.). 317-324 (1993)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kanda K., Ohmori S., Yamamoto C., Kawano S., Hayamizu S., Miyamoto N., Murata Y.and Seo H.: "Changes in circadian variations of urine excretion in tail-suspended rats." Ann.Res.Inst.Environ.Med., Nagoya Univ.43. 1-4 (1993)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kawano S., Kanda K., Murata Y., Ohmori S., Miyamoto N., Sueda K.and Hisao S.: "Endocrinological evaluations of muscle and bone atrophy in tail-suspended rats." Ann.Res.Inst.Environ.Med., Nagoya Univ.44. 5-8 (1993)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Sueda K., Imao M., Iwata, N., Kondou Y., Sugioka M., Hamaguchi C., Harada R.and Fujisawa N.: "The Effect of Tail Suspension Stress on the Vitamin A Metabolism in the Rat." J.Nagoya Women's Univ.39. 35-40 (1993)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kawano S., Ohmori S., Kanda K., Ito T., Murata Y.and Seo H.: "Adrenocortical Response to Tail-suspension in Young and old Rats." Environ.Med.38, No.1. 7-12 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito T., Ohmori S., Kanda K., Kawano S., Murata Y and Seo, H.: "Changes in Serum 1,25-Dihydroxyvitamin D_3 and mRNAs for Osteocalcin and Alkaline Phosphatase in Femur Unloaded by Tail Suspension in Rats." Environ.Med.38, No.2. 103-107 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito T., Ohmori S., Kanda K., Kawano S., Murata Y., and Seo H.: "Changes in the urinary excretions of markers for bone metabolism.-Comparison of pyridinoline and hydroxyproline-" Ann.Res.Inst.Environ.Med., Nagoya Univ.45. 12-14 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawano S., Kanda K., Ohmori S., Ito T., Murata Y., Miyamoto N.and Seo H.: "Changes in urinary excretion of corticosterone and hydroxyproline in tail suspended rats : Influenced of aging." Ann.Res.Inst.Environ.Med., Nagoya Univ.45. 15-18 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawano S., Kanda K., Ohmori S., Murata Y.and Seo H.: "Influence of aging on stress reaction : Changes in cathecholamine secretion during tail suspension in rats." Ann.Res.Inst.Environ.Med., Nagoya Univ.46 (in press). (1995)

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Published: 1996-04-15  

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