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1993 Fiscal Year Final Research Report Summary

Design of a Molecule that Generates Hydroxyl Radical upon Longer Wavelength Photoirradaition -Development of "Photo-Fenton" Reagent

Research Project

Project/Area Number 04805087
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Synthetic chemistry
Research InstitutionTOYAMA UNIVERSITY

Principal Investigator

MATSUGO Seiichi  Faculty of Engineering, Toyama University, associate professer, 工学部, 助教授 (30148126)

Project Period (FY) 1992 – 1993
KeywordsHydroxyl Radical / Longer-wavelength Photoirradiation / DNA Cleavage / DNA Modification / Protein Damage / Transfection Efficiency / 酸化的損傷
Research Abstract

The compound recognizing and cleaving DNA has widely been explored in connection with the artificial nucleases. We've been interested in designing the compounds that generate specific oxygen radicals such as hydroxyl radical upon longer wavelength photoirradiation (>350 nm). Using this type of compound, we can evaluate stoichiometric DNA lesion by the hydroxyl radical. In this line of study, we prepared a series of compounds that generate hydroxyl radical upon longer wavelength photoirradiation. Among them, a compound refered to "Photo-Fenton" reagent showed the most effective DNA cleaving activity and the cleavage occurred specifically at -GG site of DNA.The yield of 8-hydroxydeoxyguanosine (8-OHdG) from the reaction of calf thymus DNA and "photo-Fenton" reagent was 1.1% at most. Even at low concentration of "Photo-Fenton" reagent, the formation of 8-0HdG was observed . The transfecting activity of fx 174 DNA to E.coli was significantly decreased upon photoirradiated with "Photo-Fenton" reagent. The reaction of "Photo-Fenton" reagent with protein was also examined using crystallins. As a result, aggregation of the protein and significant decrease of tryptophan residue of the protein was observed. From these studies, we can evaluate the initial damage of DNA nad protein by using "Photo-Fenton" reagent.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] P.Guptasarma: "Hydroxyl Radical Mediated Damage to Proteins,with Special Reference to the Crystallins" Biochemistry. 31. 4296-4303 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Matsugo: "Significant Formation of 8-Hydroxydeoxyguanosine in Photoirradiation of“Photo-Fenton"Reagent'with calf thymus DNA and 45178i Cells." Chem.Lett.,. 453-456 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Matsugo: "Inhibitory Effects on the Cell Growth of Acanthamoeha by the hydroperoxynaphthalimide Derivatives Upon photoirradiation" Photomed.Photobiol.14. 95-96 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Matsugo: "Transtecting Activity of Photoirradiated PX174 DNA in the Presence of Hydroperoxynaphthalimides" Bioorg.Med.Chem.Lett.,. 3. 1671-1674 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Matsugo: "DNA Strand Scission and Cell-hilling Activity Observed in Photoirradiation of Hydroperoxynaphthalimide Derivatives" Excerpta Medica“Oxygen Radicals". 111-114 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松郷誠一: "光活性化薬剤によるAcanthamoehaの増殖阻害" 日本原生動物学会誌. 27. 34-35 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Matsugo(分担執筆: "Organic Peroxides" John Willey &.Sons, 845 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] P.Guptasarma, D.Balasubramanian, S.Matsugo, and I.Saito: "Hydroxyl Radical Mediated Damage to Proteins, with Special Reference to the Crystallins" Biochemistry. 31. 4296-4303 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Matsugo, S.Kumaki, C.Shimasaki, T.Mori, and I.Saito: "Significant Formation of 8-Hydroxydeoxyguanosine in Photoirradiation of "Photo-Fenton" Reagent with Calf Thymys DNA and L 5178Y Cells" Chem.Lett.453-456 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Matsugo, Y.Takehara, T.Tsuruhara, and I.Saito: "Inhibitory Effects on the Cell Growth of Acanthoamoeba by the Hydroperoxynaphthalimide Derivatives upon Photoirradiation" Photomed. Photobiol. 14. 95-96 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Matsugo, K.-I.Kodaira, and I.Saito: "Transfecting Activity of Photoirradiated fx 174 DNA in the Presence of Hydroperoxynaphthalimides" Bioorg.Med.Chem.Lett., 3. 1671-1674 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Matsugo, T.Tsuruhara, and I.Saito: "DNA Strand Scission and Cell-killing Activity Observed in Photoirradiation of Hydroperoxynaphthalimide Derivatives" Excerpta Medica "Oxygen Radicals". 111-114 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Matsugo, R.Takeuchi, S.Matsunaga, and T.Tsuruhara: "Growth Inhibition on Acanthamoeba by Light-activated Reagents" Jpn.J.Protozool.27. 34-35 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Matsugo, and I.Saito: "Dialkyl Peroxides" "Oxygen Radicals" John-Willey & Sons. 157-194 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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