1993 Fiscal Year Final Research Report Summary
Isotation and analysis of symptom expression genes in virus-infected by transposon-tagging method.
| Project/Area Number |
04806005
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物保護
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| Research Institution | Tohoku University |
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Principal Investigator |
TAKAHASHI Hideki Faculty of Agriculture Tohoku University, Instructor, 農学部, 助手 (20197164)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Symptom expression / Cucumber mosaic virus / Gene tagging / Arabidopsis |
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| Research Abstract |
To investigate the genetic determinants of severe chlorotic spot symptoms in cucumber mosaic virus(CMV[Y])-inoculated tobacco(Nicotiana tabacum'Ky57')leaves, symptom expression was compared among tobaccos(N.tabacum'Ky57', N.tabacum'Xanthi nc', and their hybrids)inoculated with CMV(Y), CMV(O), or pseudorecombinants of both strains of CMV.Xanthi nc showed very mild chlorotic spot shmptoms in CMV(Y)-inoculated leaves. Both CMV(O)-inoculated Ky57 and Xanthi nc leaves showed no symptoms. In pseudorecombinant-inoculated Ky57 leaves, severe symptoms were determined by chimeric CMV(O)and CMV(Y)RNA3 transcripts, which contained most of the coat protein gene of CMV(Y). Genetic analysis of severe sympotom formation in CMV(Y). or pseudorecombinantinoculated tobaccos indicated that two recessive nuclearcoded host genes determined severe symptom formation in CMV(Y)-inoculated Ky57. The two recessive host genetic loci completely cosegregated with the two recessive burley loci(yb1 and yb2 genes)of Ky57, whose character is an apparent reduction in chlorophyll of the stems. When several cultivars of N.tabacum were inoculated with CMV(Y), all burley tobaccos clearly showed severe symptoms, but most other cultivars showed very mild symptoms, These results suggest that severe chlorotic spot symptom formation in CMV(Y)-inoculated Ky57 is induced by a combination of the virus coat protein gene and two recessive host genes that are closely linked to or identical to yb genes.
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