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1994 Fiscal Year Final Research Report Summary

RESEARCH FOR EXPRESSION MECHANISMS OF BROAD SPECTRUM PESISTANCE OF TRANSGENIC PLATS AGAINST VIRUS DESEASES

Research Project

Project/Area Number 04806007
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

NAMBA Shigetou  THE UNIVERSITY OF TOKYO,DEPARTMENT OF AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (50189221)

Project Period (FY) 1992 – 1994
Keywordscustom PCR / genus Potyvirus / coat protein mediated protection / Nicotiana benthamiana
Research Abstract

Coat protein (CP) genes ofseveral isolates of CMV and many species of genus Potyvieus were engineered and transferred into tobacco and Nicotiana benthamiana by custom PCR (CPCR) engineering. Transformed plants gave high livels of CP expression and exhibited broad form coat protein mediated resistance to potyviruses. This suggests the feasibility of using a limited number of potyviruses to get protection against a broad range of potyviruses.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Candresse,T.: "Family description:Trichovirus.In classification and nomenculture of viruses,virus report." Archives of viralogy. (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Namba,S.: "Familly description:Capillovirus.On classification and nomenculture of viruses with report." Archives of Virology. (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Martelli,G.P.: "Trichovirus,a new genus of plant viruses" Archives of Virology. 134. 451-455 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Gonsalves,C.: "Transfer of cucumber mosaic virus-white leaf strain coat protein gene into Cucumis melo by A.tumefaciens and microprojectiles and evaltuation of tranagenic plants for protection against CMV infections." J.Amer.Soc.Hort.Sci.119(2). 345-355 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohira,K.: "Nucleotide sequence of the 3'-terminal region of citrus tatter leaf virus RNA." Virus Genes. 8. 169-172 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohira,K.: "Nucleotide sequence of the coat protein coding region of tulip breaking virus." Viirus Genes. 8. 165-167 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hidaka,M.: "Cloning and sequencing of the 3' half of a potato virus Y (0 strain) genome encoding the 5k protein protease,polymerase and coat protein." Nuc.Acids Res.20. 3515 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Namba,S.: "Protecition of transgenic plants expressing the coat protein geme of watermelon mosaic virus II or zucchini yellow mosaic against six potyviruses." Phytopatholgy. 82(9). 940-946 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ochi,M.: "Nucleotide sequence of the 3'-terminal region of potato virus tRNA." Ann.Phytopath.Soc.Japan. 58(3). 416-425 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Candresse, T.: "Family description : Trichovirus. In Classification and Nomenclature of Viruses, VIth Report." Archives of Virology (Supplementum). (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Namba, S.: "Family description : Capillovirus. In Classification and Nomenclature of Viruses, VIth Report." Archives of Virology (Supplementum). (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Martelli, G.P.: "Trichovirus, a new genus of plant viruses" Archives of Virology. 134. 451-455 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gonsalves, C.: "Transfer of cucumber mosaic virus-white leaf strain coat protein gene into Cucumis melo by A.tumefaciens and microprojectiles and evaluation of transgenic plants for protection against CMV infections." J.Amer.Soc.Hort.Sci.119 (2). 345-355 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohira, K.: "Nucleotide sequence of the 3'-terminal region of citrus tatter leaf virus RNA." Virus Genes. 8. 169-172 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohira, K.: "Nucleotide sequence of the coat protein coding region of tulip breaking virus." Virus Genes. 8. 165-167 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hidaka, M.: "Cloning and sequencing of the 3' half of a potato virus Y (O strain) genome encoding the 5k protein, protease, polymerase and coat protein." Nuc.Acids Res.20. 3515 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Namba, S.: "Protection of transgenic plants expressing the coat protein gene of watermelon mosaic virus II or zucchini yellow mosaic virus against six potyviruses." Phytopathology. 82 (9). 940-946 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ochi, M.: "Nucleotide sequence of the 3'-terminal region of potato virus T RNA." Ann.Phytopath.Soc.Japan. 58 (3). 416-425

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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