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1993 Fiscal Year Final Research Report Summary

Short-and long-term effects of cytolines on the body water homeostasis-studies in conscious rats

Research Project

Project/Area Number 04807010
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 環境生理学(含体力医学・栄養生理学)
Research InstitutionMiyazaki Medical College

Principal Investigator

KANNAN Hiroshi  Miyazaki Med.College, Dept.Physiol., Professor, 医学部, 教授 (00049058)

Project Period (FY) 1992 – 1993
KeywordsInterleukin 1beta / cardiovascular system / body water homeostasis / drinking / kidney / urinary electrolyte excretion / autonomic nervous system / conscious rat
Research Abstract

To elucidate the roles of interleukin 1 beta(IL-1beta), a cytokine with diverse actions, in the control of the cardiocascular system and body fluid balance, the effects of IL-1beta on drinking behavior, cardiovascular system and renal functions were examined in conscious rats.
(1) Drinking behavior -- IL-1beta (i.p.) attenuated osmotic thirst and spontaneous drinking, while hypovolemic thirst and angiotensin II - induced drinking were not affected. The inhibitory responses were suppressed by pretreatment with indomethacin, a cytooxygenase inhibitor.
(2) Cardiovascular function -- IL-1beta (i.v.and i.c.v.) elicited increases in arterial blood pressure (AP), heart rate (HR) and renal sympathetic nerve activity (RSNA )accompained by an increase in body terperature, which were blocked by pretreatment with indomethacin. The maximum changes in AP, HR and RSNA occurred 10 - 15 min after i.v.injection of IL-1beta (100ng), and 20 - 25 min after i.c.v.injection (5 ng). The activity of the sympathe … More tic efferent nerves innervating the interscapular brown adipose tissue (BAT) was also increased by IL-1beta. Intravenous injection of IL-1beta increased plasma nosradrenaline and ACTH concentrations. Thus, IL-1beta produces simultaneously activation of sympathetic outflow and ACTH release.
(3) Renal function --IL-1beta (i.p.) decreased daily urinary sodium and potassium excretion, while urine volume was not affected. The IL-1beta - induced decrease in sodium excretion was abolished in renal denervated rats. Kainic acid was injected into the anterioventral third ventricle (AV3V) region, including the organum vasculosum of the lamina terminalis, to examine whether neurons in the AV3V region are involved in the IL-1beta induced responses. The IL-1beta induced decreases in urinary sodium and potassium excretion were abolished inrats with a kainic acid - induced lesion in the AV3V region, while the suppression in drinking and food intake was attenuated but still persistent. Thus, there appears to be several anatomically distinct sites of action of IL-1beta within the brain, possibly involving different receptor subtypes and mechanisms. Less

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Osaka,T.: "Intraperitoneal administration of recombinant human interleukin-1β inhibits osmotic thirst in the rat." Physiol.Behav.51. 1267-1270 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Li,Z.: "Interleukin-1β directly excites hypothalamic supraoptic neurons in rats in vitro." Neuro Report. 3. 91-93 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamashita,H.: "Inhibition of hypothalamic neurons by the atrial natriuretic peptide family." NIPS. 7. 75-79 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto,S.: "The actions of endothelin on single cells in the anteroventral third ventricular region and supraoptic nucleus in rats." J.Neuroendocrinol.5. 427-434 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ueta,Y.: "CCK-8 excites oxytocin-secreting neurons in the paraventricular nucleus in rats-possible involvement of noradrenergic pathway." Brain Res.32. 453-459 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kannan,H.: "Effects of centrally administered endothelin-3 on renal sympathetic nerve activity and renal blood flow in conscious rats." J.Auton.Nerv.Syst.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osaka, T., Kannan, H., Kawano, S., Ueta, Y.& Yamashita, H.: "Intraperitoneal administration of recombinant human interleukin-1beta inhibits osmotic thirst in the rat." Physiol.Behav.51. 1267-1270 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Li, Z., Inenaga, H., Kawano, S., Ueta, Y.& Yamashita, H.: "Interleukin-1beta directly excites hypothalamic supraoptic neurons in rats in vitro." Neuro Report. 3. 91-93 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamashita, H.& Kannan, H.: "Inhibition of hypothalamic neurons by the atrial natriuretic peptide family" NIPS. 7. 75-79 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, S., Inenaga, K., Kannan, H., Eto, S.& Yamashita, H.: "The actions of endothelin on single cells in the anteroventral third centricular region and supraoptic nucleus in rats." J.Neuroendocrinol.5. 427-434 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueta, Y., Kanna, H., Higuchi, T., Negoro, H.& Yamashita, H.: "CCK-8 excites oxytocin-secreting neurons in the paraventricular nucleus in rats-possible involvement of noradrenergic pathway." Brain Res.32. 453-459 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kannan, H., Tanaka, Y., Ueta, Y., Hayashida, Y.& Yamashita, H.: "Effects of centrally administered endothelin-3 on renal sympathetic nerve activity and renal blood flow in conscious rats." J.Auton.Nerv.Syst.(in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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