1994 Fiscal Year Final Research Report Summary
Establishment of the Analytical Techniques for Various Chemicals in Human Tissues
| Project/Area Number |
04807042
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
KUDO Keiko Kyushu Univ.Fac.of Med., Research Assoc., 医学部, 助手 (10186405)
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| Co-Investigator(Kenkyū-buntansha) |
JITSUFUCHI Narumi Kyushu Univ.Fac.of Med., Research Assoc., 医学部, 助手 (80232916)
IMAMURA Tohru Kyushu Univ.Fac.of Med., Lecturer, 医学部, 講師 (00193681)
KIMURA Kojiro Shimane Med.Univ., Professor, 医学部, 教授 (30153191)
NAGATA Takeaki Kyushu Univ.Fac.of Med., Professor, 医学部, 教授 (00078586)
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| Project Period (FY) |
1992 – 1994
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| Keywords | poisonong / triazolam / postmortem change / GC / paraquat / diquat / HPLC |
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| Research Abstract |
A reliable and sensitive method has been developed to assess the concentrations of the hypnotic drug triazolam in human tissues, including putrefied tissues. A forensic study was performed on the toxicological effects of triazolam using putrefied tissues.
Postmortem changes of triazolam concentrations in body tissues were examined using rats, in order to find the most suitable tissue samples for toxicological analysis. Triazolam concentrations were markedly increased in the spleen, the abdominal muscle, the liver and the kindey, and were slightly increased in the blood and the lung. On the other hand, no changes were observed in the thigh muscle or the brain, and the value in the thigh muscle was similar to those in the blood samples collected immediately after death. The results indicate that triazolam diffuses into the surrounding tissues through the stomach wall after death, so that the thigh muscle and the brain have to be analyzed as well as the blood for a correct diagnosis of triazolam ingestion.
A simple, sensitive, reliable, and economical method for simultaneous determination of paraquat dichloride and diquat dibromide in human biological materials was developed, using high performance liquid chromatography. We used this method to examine the concentrations of paraquat and diquat in tissues of an autopsied individual.
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