1993 Fiscal Year Final Research Report Summary

Immunological Regulation and Modulation by LIF

Research Project

Project/Area Number	04807087
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	General surgery
Research Institution	Keio University
Principal Investigator	ENDO Masao Keio University Dept.Surg, Instructor, 医学部, 講師 (00112681)
Co-Investigator(Kenkyū-buntansha)	YAMATAKA Koichi Keio University Dept.Surg. Instructor, 医学部, 助手 (10230442) IIO Hiroshi Keio University Dept.Surg. Instructor, 医学部, 助手 (20202806)
Project Period (FY)	1992 – 1993
Keywords	LIF / SEKI / colon 26 / Eosiocyte / cytokine
Research Abstract	The disturbances of metabolism and immunosystem were commonly observed in human cancer cachexia. The abnormality of immunosystem was examined in cachexic nude rats transplanted cancer cell lines. Mice colon cell line colon and human melanoma cell line SEKI which induce severe cachexia were transplanted s.c. to the bilateral flanks of nude rats. The blood samples were collected from peripheral vein on 3 weeks after transplantation. White blood cell, eosinocyte, and eosinocyte percentage were counted. Suppression of eosinophil differentiation activity in vitro was investigated by useing and eosinophil peroxydase assay. Eosinocyte percentage was 0% in nude rats transplanted colon 26 and SEKI, which 11% in controls(p<0.05). Lecocytosis was observed only in nude rats with colon 26 but not SEKI and control. Eosinophil differentiation activity was remarkable suppressed by the medium clutered with colon 26 and SEKI.These data mean that colon 26 and SEKI produce the anti-differentiated substance for eosinocyte.