1993 Fiscal Year Final Research Report Summary
Elucidation of genetic disorders in skeletal dysplasias.
| Project/Area Number |
04807105
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
NAKAHARA Haruhiko Osaka Uni.Med.School, Orthopaedics, Assist Professor, 医学部, 助手 (10198179)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ONO Keiro Osaka Uni.Med.School, Orthopaedics, Professor, 医学部, 教授 (70028330)
KIMURA Tomoatsu Osaka Uni.Med.School, Orthopaedics, Associal Professor, 医学部, 講師 (80167379)
|
|---|
| Project Period (FY) |
1992 – 1993
|
| Keywords | periosteum / bone / cartilage / differentiation / cytokines |
|---|
| Research Abstract |
Periosteum-derived cells have been reported to exhibit osteogenic and chondrogenic potentials in high cell density culture conditions. Using this system, the effects of TGFbeta and BMP-2 on proliferation and differentiation of periosteal mesenchymal cells were studied. TGFbeta shortened the time course of chondrogenesis and increased the amount of cartilage formation. On the other hand, the amount of bone decreased with TGFbeta treatment, whereas the time course of osteogenesis remained unaffected. BMP-2 enhanced bone formation without any effect on chondrogenesis. The developmental potential of periosteum-derived cells was also clonally assessed with an agar gel culture system. Two types of colonies were predominantly observed, one type of colony being chondrogenic, the other type osteogenic colonies. Supplementation with TGFbeta to this system shortened the time course of chondrogenesis and also increased colony forming efficiency of chondrogenic colonies. On the other hand, colony forming efficiency of osteogenic colonies decreased with TGFbeta treatment. These observations suggest that there are both committed osteoprogenitor and chondroprogenitor cells present in the periostel cell population, and TGFbeta stimulates proliferation and differentiation of chondrogenic cell population by its targeted action. These resuls taken together suggest that TGFbeta and BMP regulate osteochondrogenic differrentiation of cells in the periosteum in the process of fracture healing.
|
